129-Elane^tm1(cre)Roes/H

Status	Available to order
EMMA ID	EM:00075
International strain name	129-Elane^tm1(cre)Roes/H
Alternative name	GEcre
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Elane^tm1(cre)Roes
Gene/Transgene symbol	Elane

Information from provider

Provider	Jurgen Roes
Provider affiliation	Windeyer Institute of Medical Sciences, University College London
Genetic information	Insertion of the cre recombinase at the ATG of Elane (Ela2) gene sequence.
Phenotypic information	Impaired innate immunity.
References	Impaired immunity and enhanced resistance to endotoxin in the absence of neutrophil elastase and cathepsin G.;Tkalcevic J, Novelli M, Phylactides M, Iredale J P, Segal A W, Roes J, ;2000;Immunity;12;201-10; 10714686

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Cyclic neutropenia / Orphanet_2686
Autosomal dominant severe congenital neutropenia / Orphanet_486

MGI phenotypes (allele matching)

impaired neutrophil recruitment / MGI
increased susceptibility to infection induced morbidity/mortality / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI

MGI phenotypes (gene matching)

abnormal inflammatory response / MGI
no abnormal phenotype detected / MGI
increased susceptibility to bacterial infection / MGI
abnormal neutrophil physiology / MGI
immune system phenotype / MGI
hematopoietic system phenotype / MGI
decreased tumor necrosis factor secretion / MGI
impaired neutrophil recruitment / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased susceptibility to infection induced morbidity/mortality / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI

Literature references

Impaired immunity and enhanced resistance to endotoxin in the absence of neutrophil elastase and cathepsin G.;Tkalcevic J, Novelli M, Phylactides M, Iredale J P, Segal A W, Roes J, ;2000;Immunity;12;201-10; 10714686
PD-L1 maintains neutrophil extracellular traps release by inhibiting neutrophil autophagy in endotoxin-induced lung injury.;Zhu Cheng-Long, Xie Jian, Zhao Zhen-Zhen, Li Peng, Liu Qiang, Guo Yu, Meng Yan, Wan Xiao-Jian, Bian Jin-Jun, Deng Xiao-Ming, Wang Jia-Feng, ;2022;Frontiers in immunology;13;949217; 36016930
Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels.;Hyun Young-Min, Sumagin Ronen, Sarangi Pranita P, Lomakina Elena, Overstreet Michael G, Baker Christina M, Fowell Deborah J, Waugh Richard E, Sarelius Ingrid H, Kim Minsoo, ;2012;The Journal of experimental medicine;209;1349-62; 22711877
Role of β1 integrin in tissue homing of neutrophils during sepsis.;Sarangi Pranita P, Hyun Young-Min, Lerman Yelena V, Pietropaoli Anthony P, Kim Minsoo, ;2012;Shock (Augusta, Ga.);38;281-7; 22683734
Sepsis lethality via exacerbated tissue infiltration and TLR-induced cytokine production by neutrophils is integrin α3β1-dependent.;Lerman Yelena V, Lim Kihong, Hyun Young-Min, Falkner Kathleen L, Yang Hongmei, Pietropaoli Anthony P, Sonnenberg Arnoud, Sarangi Pranita P, Kim Minsoo, ;2014;Blood;124;3515-23; 25278585
Neutrophils support lung colonization of metastasis-initiating breast cancer cells.;Wculek Stefanie K, Malanchi Ilaria, ;2015;Nature;528;413-7; 26649828
Radiation exposure elicits a neutrophil-driven response in healthy lung tissue that enhances metastatic colonization.;Nolan Emma, Bridgeman Victoria Louise, Ombrato Luigi, Karoutas Adam, Rabas Nicolas, Sewnath Celine Angeli Natascha, Vasquez Marcos, Rodrigues Felipe Silva, Horswell Stuart, Faull Peter, Carter Rebecca, Malanchi Ilaria, ;2022;Nature cancer;3;173-187; 35221334
GSDMD-mediated NETosis promotes the development of acute respiratory distress syndrome.;Xie Jian, Zhu Cheng-Long, Wan Xiao-Jian, Zhao Zhen-Zhen, Meng Yan, Li Peng, Guo Yu, Liu Qiang, Bian Jin-Jun, Deng Xiao-Ming, Wang Jia-Feng, ;2023;European journal of immunology;53;e2250011; 36250416
PD-L1 promotes GSDMD-mediated NET release by maintaining the transcriptional activity of Stat3 in sepsis-associated encephalopathy.;Zhu Cheng-Long, Xie Jian, Liu Qiang, Wang Yi, Li Hui-Ru, Yu Chang-Meng, Li Peng, Deng Xiao-Ming, Bian Jin-Jun, Wang Jia-Feng, ;2023;International journal of biological sciences;19;1413-1429; 37056920

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

129-Elanetm1(cre)Roes/H