STOCK Rag2tm1Fwa Cd40tm1Kik Tg(TcraH-Y,TcrbH-Y)71Vbo/Orl

Status

Available to order

EMMA IDEM:07463
Citation informationRRID:IMSR_EM:07463 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameSTOCK Rag2tm1Fwa Cd40tm1Kik Tg(TcraH-Y,TcrbH-Y)71Vbo/Orl
Alternative nameRag2-/- CD40-/- HY+/+Tg
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCd40tm1Kik, Tg(TcraH-Y,TcrbH-Y)71Vbo, Rag2tm1Fwa
Gene/Transgene symbolCd40, Tg(TcraH-Y,TcrbH-Y)71Vbo, Rag2

Information from provider

ProviderBenedita Rocha
Provider affiliationINSERM U 1020, INSERM
Additional ownerFrederick W. Alt, Hitoshi Kikutani, Harald von Boehmer
Genetic informationMutant mice bearing a transgenic alpha-beta TCR specific for the HY male antigen restricted to major histocompatibility complex (https://www.informatics.jax.org/allele/MGI:3044562), and deficient in the Cd40 gene and the Rag2 recombinase gene (TgRAG2) were used to obtain monoclonal populations of CD8+ T cells.
Phenotypic informationMice will have a single population of lymphocytes, those expressing the TCR transgene.
Breeding historyCurrently bred as homozygotes for the Rag2 and Cd40 knock-out mutations and the TCR transgene.
References
  • A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory.;Bourgeois Christine, Rocha Benedita, Tanchot Corinne, ;2002;Science (New York, N.Y.);297;2060-3; 12242444
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedyes

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Breeding at archiving centreMales are homozygous knock-out for Rag2 and Cd40, transgenic for the Tg(TcraH-Y,TcrbH-Y)71Vbo (HY) transgene. Females are homozygous knock-out for Rag2 and Cd40, heterozygous or wild-type for the Tg(TcraH-Y,TcrbH-Y)71Vbo (HY) transgene.
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased bone mineral density / MGI
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to bacterial infection / MGI
  • hematopoietic system phenotype / MGI
  • decreased cerebral infarction size / MGI
  • decreased platelet aggregation / MGI
  • decreased trabecular bone mass / MGI
  • decreased compact bone mass / MGI
  • decreased B cell number / MGI
  • immune system phenotype / MGI
  • absent germinal center B cells / MGI
  • decreased neutrophil cell number / MGI
  • abnormal humoral immune response / MGI
  • decreased IgA level / MGI
  • decreased immunoglobulin level / MGI
  • decreased IgE level / MGI
  • decreased susceptibility to type II hypersensitivity reaction / MGI
  • absent spleen germinal center / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
MGI phenotypes (gene matching)
  • decreased bone mineral density / MGI
  • decreased neutrophil cell number / MGI
  • abnormal humoral immune response / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to bacterial infection / MGI
  • decreased immunoglobulin level / MGI
  • abnormal immunoglobulin level / MGI
  • decreased IgE level / MGI
  • increased IgM level / MGI
  • decreased B cell number / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased susceptibility to type II hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • decreased cerebral infarction size / MGI
  • absent germinal center B cells / MGI
  • absent spleen germinal center / MGI
  • decreased spleen germinal center number / MGI
  • decreased spleen germinal center size / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • increased IgG3 level / MGI
  • abnormal lymph node germinal center morphology / MGI
  • decreased platelet aggregation / MGI
  • decreased trabecular bone mass / MGI
  • decreased compact bone mass / MGI
  • impaired humoral immune response / MGI
  • decreased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • decreased bone marrow cell number / MGI
  • alopecia / MGI
  • abnormal digestive system morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal Peyer's patch morphology / MGI
  • abnormal thymus morphology / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • decreased body size / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • lung inflammation / MGI
  • neoplasm / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • small lymph nodes / MGI
  • abnormal lymph node morphology / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal pre-B cell morphology / MGI
  • increased susceptibility to infection / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal effector T cell morphology / MGI
  • abnormal macrophage physiology / MGI
  • abnormal B cell number / MGI
  • abnormal B cell physiology / MGI
  • abnormal immune system organ morphology / MGI
  • colitis / MGI
  • absent Peyer's patches / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • erythroderma / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal B cell morphology / MGI
  • decreased lymphocyte cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • cachexia / MGI
  • increased macrophage cell number / MGI
  • abnormal T-helper 1 physiology / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • abnormal T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • absent mature B cells / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
  • decreased spleen white pulp amount / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • absent Hassall's corpuscle / MGI
  • small inguinal lymph nodes / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • increased DN3 thymocyte number / MGI
  • abnormal stomach mucosa morphology / MGI
  • small cervical lymph nodes / MGI

Literature references

  • A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory.;Bourgeois Christine, Rocha Benedita, Tanchot Corinne, ;2002;Science (New York, N.Y.);297;2060-3; 12242444

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