B6D2-Tg(Phox2b-cre)1Jbr/Orl
| Status | Available to order |
| EMMA ID | EM:07452 |
| Citation information | RRID:IMSR_EM:07452 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6D2-Tg(Phox2b-cre)1Jbr/Orl |
| Alternative name | Phox2bcre::BAC1 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Phox2b-cre)1Jbr |
| Gene/Transgene symbol | Tg(Phox2b-cre)1Jbr |
Information from provider
| Provider | Jean-Francois Brunet |
| Provider affiliation | IBENS, Ecole normale superieure |
| Genetic information | The cre ORF was fused in frame to the first 82 amino acids of mouse Phox2b by recombineering in BAC RP24-95M11. The recombined BAC was microinjected into (C57BL/6 X DBA/2)F1 blastocysts. |
| Phenotypic information | Phox2b::cre BAC transgenic mice from founder line BAC1 direct Cre recombinase expression to all cells that express or have expressed Phox2b or descend from Phox2b-expressing progenitors. In the central nervous system, these cells include the branchio-visceromotor neurons, the noradrenergic neurons, the neurons of the nucleus of the solitary tract and of the retrotrapezoid nucleus and a subpopulation of serotonergic neurons. In the peripheral nervous system, Cre expression is directed to the sympathetic, parasympathetic, cranial sensory and enteric ganglia. The Phox2b::cre mice may be used to generate conditional mutations in neurons involved in the control of blood pressure, heart rate, autonomic regulation of breathing, feeding, digestion and taste perception. |
| Breeding history | Outcrossed for at least 10 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6 x DBA/2 males |
Literature references
- Homeoprotein Phox2b commands a somatic-to-visceral switch in cranial sensory pathways.;D'Autréaux Fabien, Coppola Eva, Hirsch Marie-Rose, Birchmeier Carmen, Brunet Jean-François, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;20018-23; 22128334