B6D2-Tg(Phox2b-cre)1Jbr/Orl
| Status | Available to order |
| EMMA ID | EM:07452 |
| International strain name | B6D2-Tg(Phox2b-cre)1Jbr/Orl |
| Alternative name | Phox2bcre::BAC1 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Phox2b-cre)1Jbr |
| Gene/Transgene symbol | Tg(Phox2b-cre)1Jbr |
Information from provider
| Provider | Jean-Francois Brunet |
| Provider affiliation | IBENS, Ecole normale superieure |
| Genetic information | The cre ORF was fused in frame to the first 82 amino acids of mouse Phox2b by recombineering in BAC RP24-95M11. The recombined BAC was microinjected into (C57BL/6 X DBA/2)F1 blastocysts. |
| Phenotypic information | Phox2b::cre BAC transgenic mice from founder line BAC1 direct Cre recombinase expression to all cells that express or have expressed Phox2b or descend from Phox2b-expressing progenitors. In the central nervous system, these cells include the branchio-visceromotor neurons, the noradrenergic neurons, the neurons of the nucleus of the solitary tract and of the retrotrapezoid nucleus and a subpopulation of serotonergic neurons. In the peripheral nervous system, Cre expression is directed to the sympathetic, parasympathetic, cranial sensory and enteric ganglia. The Phox2b::cre mice may be used to generate conditional mutations in neurons involved in the control of blood pressure, heart rate, autonomic regulation of breathing, feeding, digestion and taste perception. |
| Breeding history | Outcrossed for at least 10 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6 x DBA/2 |
Literature references
- Homeoprotein Phox2b commands a somatic-to-visceral switch in cranial sensory pathways.;D'Autréaux Fabien, Coppola Eva, Hirsch Marie-Rose, Birchmeier Carmen, Brunet Jean-François, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;20018-23; 22128334