C57BL/6NTac-Pkn1tm1a(KOMP)Wtsi/H

Status

Available to order

EMMA IDEM:07366
International strain nameC57BL/6NTac-Pkn1tm1a(KOMP)Wtsi/H
Alternative nameEPD0132_5_D06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPkn1tm1a(KOMP)Wtsi
Gene/Transgene symbolPkn1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from KOMP ES clone EPD0132_5_D06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased neutrophil cell number / IMPC
  • decreased red blood cell distribution width / IMPC
  • decreased large unstained cell number / IMPC
  • decreased lymphocyte cell number / IMPC
  • decreased leukocyte cell number / IMPC
  • increased total body fat amount / IMPC
  • decreased monocyte cell number / IMPC
  • decreased fasting circulating glucose level / IMPC
  • increased hemoglobin content / IMPC
MGI phenotypes (gene matching)
  • no abnormal phenotype detected / MGI
  • increased IgM level / MGI
  • glomerulonephritis / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • abnormal somatic hypermutation frequency / MGI
  • increased B cell proliferation / MGI
  • immune system phenotype / MGI
  • increased germinal center B cell number / MGI
  • abnormal germinal center B cell physiology / MGI
  • increased spleen germinal center size / MGI
  • increased IgG1 level / MGI
  • increased IgG2a level / MGI
  • increased IgG2b level / MGI
  • increased splenocyte number / MGI
  • decreased activation-induced B cell apoptosis / MGI
  • increased renal glomerulus basement membrane thickness / MGI
  • mesangial cell hyperplasia / MGI
  • renal glomerular immunoglobulin deposits / MGI

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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