C57BL/6NTac-Pkn1^{tm1a(KOMP)Wtsi}/H

Status	Available to order
EMMA ID	EM:07366
Citation information	RRID:IMSR_EM:07366 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Pkn1^{tm1a(KOMP)Wtsi}/H
Alternative name	EPD0132_5_D06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Pkn1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Pkn1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from KOMP ES clone EPD0132_5_D06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased neutrophil cell number / IMPC
decreased red blood cell distribution width / IMPC
decreased large unstained cell number / IMPC
decreased lymphocyte cell number / IMPC
decreased leukocyte cell number / IMPC
increased total body fat amount / IMPC
decreased monocyte cell number / IMPC
decreased fasting circulating glucose level / IMPC
increased hemoglobin content / IMPC

MGI phenotypes (gene matching)

no abnormal phenotype detected / MGI
increased IgM level / MGI
glomerulonephritis / MGI
increased anti-double stranded DNA antibody level / MGI
abnormal somatic hypermutation frequency / MGI
increased B cell proliferation / MGI
immune system phenotype / MGI
increased germinal center B cell number / MGI
abnormal germinal center B cell physiology / MGI
increased spleen germinal center size / MGI
increased IgG1 level / MGI
increased IgG2a level / MGI
increased IgG2b level / MGI
increased splenocyte number / MGI
decreased activation-induced B cell apoptosis / MGI
increased renal glomerulus basement membrane thickness / MGI
mesangial cell hyperplasia / MGI
renal glomerular immunoglobulin deposits / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Pkn1tm1a(KOMP)Wtsi/H