C57BL/6N-A^tm1Brd Usp21^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:07280
International strain name	C57BL/6N-A^tm1Brd Usp21^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0716_10_H09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Usp21^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Usp21
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0716_10_H09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation.;Pannu Jaspreet, Belle Jad I, Förster Michael, Duerr Claudia U, Shen Shiyang, Kane Leanne, Harcourt Katherine, Fritz Jörg H, Clare Simon, Nijnik Anastasia, ;2015;PloS one;10;e0117304; 25680095

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

IMPC phenotypes (allele matching)

increased leukocyte cell number / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased blood urea nitrogen level / IMPC
decreased circulating fructosamine level / IMPC

IMPC phenotypes (gene matching)

decreased circulating fructosamine level / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased blood urea nitrogen level / IMPC
increased leukocyte cell number / IMPC

MGI phenotypes (gene matching)

increased neutrophil cell number / MGI
enlarged spleen / MGI
decreased susceptibility to viral infection / MGI
no phenotypic analysis / MGI
increased spleen weight / MGI
homeostasis/metabolism phenotype / MGI
hematopoietic system phenotype / MGI
increased macrophage cell number / MGI
increased circulating interferon-alpha level / MGI
increased circulating interferon-beta level / MGI
abnormal interferon level / MGI
increased splenocyte number / MGI
decreased susceptibility to viral infection induced morbidity/mortality / MGI

Literature references

Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation.;Pannu Jaspreet, Belle Jad I, Förster Michael, Duerr Claudia U, Shen Shiyang, Kane Leanne, Harcourt Katherine, Fritz Jörg H, Clare Simon, Nijnik Anastasia, ;2015;PloS one;10;e0117304; 25680095
Ablation of USP21 in skeletal muscle promotes oxidative fibre phenotype, inhibiting obesity and type 2 diabetes.;Kim Ayoung, Koo Ja Hyun, Jin Xing, Kim Wondong, Park Shi-Young, Park Sunghyouk, Rhee Eugene P, Choi Cheol Soo, Kim Sang Geon, ;2021;Journal of cachexia, sarcopenia and muscle;12;1669-1689; 34523817

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Usp21tm1a(EUCOMM)Wtsi/WtsiBiat