C57BL/6N-A^tm1Brd Cyba^{tm1a(EUCOMM)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:07275
International strain name	C57BL/6N-A^tm1Brd Cyba^{tm1a(EUCOMM)Wtsi}/WtsiIeg
Alternative name	EPD0372_5_B08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cyba^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cyba
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0372_5_B08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Chronic granulomatous disease / Orphanet_379

IMPC phenotypes (allele matching)

increased leukocyte cell number / IMPC
persistence of hyaloid vascular system / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
abnormal gait / IMPC
stereotypic behavior / IMPC
trunk curl / IMPC
impaired righting response / IMPC
increased mean platelet volume / IMPC
increased lean body mass / IMPC
decreased blood urea nitrogen level / IMPC
decreased circulating amylase level / IMPC

IMPC phenotypes (gene matching)

impaired righting response / IMPC
stereotypic behavior / IMPC
decreased blood urea nitrogen level / IMPC
persistence of hyaloid vascular system / IMPC
decreased circulating amylase level / IMPC
increased lean body mass / IMPC
cataract / IMPC
abnormal gait / IMPC
increased mean platelet volume / IMPC
trunk curl / IMPC
increased leukocyte cell number / IMPC
abnormal lens morphology / IMPC

MGI phenotypes (gene matching)

impaired swimming / MGI
impaired balance / MGI
increased inflammatory response / MGI
lung inflammation / MGI
increased susceptibility to bacterial infection / MGI
abnormal neutrophil physiology / MGI
absent otoliths / MGI
absent linear vestibular evoked potential / MGI
sepsis / MGI
head tilt / MGI
hearing/vestibular/ear phenotype / MGI
abnormal intestinal goblet cell physiology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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