B6.129P2-Pecam1^tm1Mak/Cnbc

Status	Available to order
EMMA ID	EM:07243
International strain name	B6.129P2-Pecam1^tm1Mak/Cnbc
Alternative name	CD31/Pecam1
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Pecam1^tm1Mak
Gene/Transgene symbol	Pecam1

Information from provider

Provider	Tak W Mak
Provider affiliation	Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute
Genetic information	A targeting vector was designed to disrupt exon 7 by inserting a pGK1neo resistance expression cassette in reverse orientation of CD31 transcription.
Phenotypic information	Pecam1 (CD31)-deficient mice (CD31KO) are viable and born at the expected Mendelian frequency, remain healthy, and exhibit no obvious vascular developmental defects. In response to inflammatory challenge, polymorphonuclear leukocytes of CD31KO mice are arrested between the vascular endothelium and the basement membrane of inflammatory site mesenteric microvessels, confirming a role for CD31 in the migration of neutrophils through the subendothelial extracellular matrix.
Breeding history	Backcrossed 10 times to C57BL/6.
References	Generation and characterization of the Anp32e-deficient mouse.;Reilly Patrick T, Afzal Samia, Wakeham Andrew, Haight Jillian, You-Ten Annick, Zaugg Kathrin, Dembowy Joanna, Young Ashley, Mak Tak W, ;2010;PloS one;5;e13597; 21049064 Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions.;Duncan G S, Andrew D P, Takimoto H, Kaufman S A, Yoshida H, Spellberg J, de la Pompa J L, Elia A, Wakeham A, Karan-Tamir B, Muller W A, Senaldi G, Zukowski M M, Mak T W, ;1999;Journal of immunology (Baltimore, Md. : 1950);162;3022-30; 10072554
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)

increased susceptibility to induced arthritis / MGI
abnormal leukocyte physiology / MGI
joint inflammation / MGI
increased interferon-gamma secretion / MGI
kidney vascular congestion / MGI
liver inflammation / MGI
abnormal cytokine secretion / MGI
increased vascular permeability / MGI
pulmonary edema / MGI
pulmonary vascular congestion / MGI
liver vascular congestion / MGI
spleen vascular congestion / MGI
glomerular capillary congestion / MGI
abnormal lung morphology / MGI
impaired lung alveolus development / MGI
abnormal pulmonary alveolar sac morphology / MGI
abnormal pulmonary alveolus wall morphology / MGI
decreased lung endothelial cell migration / MGI

MGI phenotypes (gene matching)

kidney vascular congestion / MGI
abnormal lung morphology / MGI
abnormal vasodilation / MGI
liver inflammation / MGI
abnormal leukocyte physiology / MGI
joint inflammation / MGI
abnormal cytokine secretion / MGI
increased vascular permeability / MGI
increased susceptibility to induced arthritis / MGI
pulmonary edema / MGI
abnormal nitric oxide homeostasis / MGI
decreased vasodilation / MGI
impaired lung alveolus development / MGI
increased interferon-gamma secretion / MGI
pulmonary vascular congestion / MGI
liver vascular congestion / MGI
spleen vascular congestion / MGI
abnormal pulmonary alveolar sac morphology / MGI
abnormal pulmonary alveolus wall morphology / MGI
decreased lung endothelial cell migration / MGI
glomerular capillary congestion / MGI

Literature references

Generation and characterization of the Anp32e-deficient mouse.;Reilly Patrick T, Afzal Samia, Wakeham Andrew, Haight Jillian, You-Ten Annick, Zaugg Kathrin, Dembowy Joanna, Young Ashley, Mak Tak W, ;2010;PloS one;5;e13597; 21049064
Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions.;Duncan G S, Andrew D P, Takimoto H, Kaufman S A, Yoshida H, Spellberg J, de la Pompa J L, Elia A, Wakeham A, Karan-Tamir B, Muller W A, Senaldi G, Zukowski M M, Mak T W, ;1999;Journal of immunology (Baltimore, Md. : 1950);162;3022-30; 10072554

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6.129P2-Pecam1tm1Mak/Cnbc