IMPC phenotypes (gene matching)
- decreased respiratory quotient / IMPC
B6.Cg-Cdkl5tm1.1Cogr/Cnrm
| Status | Available to order |
| EMMA ID | EM:07213 |
| Citation information | RRID:IMSR_EM:07213 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Cdkl5tm1.1Cogr/Cnrm |
| Alternative name | Cdkl5cKO B6N |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Cdkl5tm1.1Cogr |
| Gene/Transgene symbol | Cdkl5 |
Information from provider
| Provider | Cornelius Gross |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Additional owner | Dr. Elena Amendola, EMBL Mouse Molecular Unit, Monterotondo, Italy |
| Genetic information | Cdkl5 exon4 was targeted by homologous recombination. Exon 4 was flanked by two loxP sites followed by neomycin cassette flanked by Flp sites used for removal of the cassette. |
| Phenotypic information | Heterozygous and homozygous conditional Cdkl5 mice do not show any Rett-like phenotype. The conditional CDKL5 mice could be considered like wild-type mice. Upon crossing these mice with a different Cre strain it is possible to generate mice with a Cdkl5 specific inactivation. |
| Breeding history | Mutant mice were crossed with C57BL/6J FLP-deleter mice (Farley et al., 2000) made congenic in B6N genetic background (N>10). After that mice were bred for 7 generations in a C57BL/6N genetic background. The first mating (FLP) was not considered in the calculation of the generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- CDKL5-related epileptic encephalopathy / Orphanet_505652
- Early infantile epileptic encephalopathy / Orphanet_1934
- Atypical Rett syndrome / Orphanet_3095
- West syndrome / Orphanet_3451
Literature references
- Mapping pathological phenotypes in a mouse model of CDKL5 disorder.;Amendola Elena, Zhan Yang, Mattucci Camilla, Castroflorio Enrico, Calcagno Eleonora, Fuchs Claudia, Lonetti Giuseppina, Silingardi Davide, Vyssotski Alexei L, Farley Dominika, Ciani Elisabetta, Pizzorusso Tommaso, Giustetto Maurizio, Gross Cornelius T, ;2014;PloS one;9;e91613; 24838000