MGI phenotypes (allele matching)
STOCK Cers6tm1.1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:07170 |
| Citation information | RRID:IMSR_EM:07170 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Cers6tm1.1Kwi/Cnrm |
| Alternative name | CerS6KILacZ |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cers6tm1.1Kwi |
| Gene/Transgene symbol | Cers6 |
Information from provider
| Provider | Klaus Willecke |
| Provider affiliation | Molecular genetics, LIMES Institute, University of Bonn |
| Genetic information | A stop codon was inserted in the coding region of exon 7 followed by an IRES-LacZ reporter cassette. A FRT-flanked neo cassette was inserted upstream of exon 8. Flp-mediated recombination removed the neo cassette. LacZ expression is detected by staining with X-Gal. |
| Phenotypic information | Behavioral impairment, altered sphingolipid metabolism. |
| Breeding history | Backcrossed to 87.5% C57BL/6. There occur no problems with homozygous breedings. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal hippocampus morphology / MGI
- hyperactivity / MGI
- impaired coordination / MGI
- limb grasping / MGI
- decreased inflammatory response / MGI
- abnormal lipid homeostasis / MGI
- increased vertical activity / MGI
- decreased circulating insulin level / MGI
- increased energy expenditure / MGI
- improved glucose tolerance / MGI
- behavior/neurological phenotype / MGI
- decreased susceptibility to diet-induced obesity / MGI
- decreased circulating leptin level / MGI
- enhanced lipolysis / MGI
- nervous / MGI
- microgliosis / MGI
- decreased brown fat cell lipid droplet size / MGI
- decreased fat cell size / MGI
- decreased total body fat amount / MGI
- abnormal cellular respiration / MGI
- abnormal habituation to a new environment / MGI
- abnormal ceramide level / MGI
- increased fatty acid oxidation / MGI
Literature references
- Inactivation of ceramide synthase 6 in mice results in an altered sphingolipid metabolism and behavioral abnormalities.;Ebel Philipp, Vom Dorp Katharina, Petrasch-Parwez Elisabeth, Zlomuzica Armin, Kinugawa Kiyoka, Mariani Jean, Minich David, Ginkel Christina, Welcker Jochen, Degen Joachim, Eckhardt Matthias, Dere Ekrem, Dörmann Peter, Willecke Klaus, ;2013;The Journal of biological chemistry;288;21433-21447; 23760501