MGI phenotypes (allele matching)
B6N.Cg-Piwil2tm2.2Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:07098 |
| Citation information | RRID:IMSR_EM:07098 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N.Cg-Piwil2tm2.2Doca/Cnrm |
| Alternative name | MiliNull |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Piwil2tm2.2Doca |
| Gene/Transgene symbol | Piwil2 |
Information from provider
| Provider | Donal O'Carroll |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | Exon 21 was flanked by two loxP sites for the formation of a null allele through Cre recombination. |
| Phenotypic information | Male sterility due to a meiotic arrest, transposons derepression due to DNA-methylation defect. |
| Breeding history | The mouse was backcrossed 8 times with C57BL/6N mice. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (gene matching)
Literature references
- Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis.;Di Giacomo Monica, Comazzetto Stefano, Saini Harpreet, De Fazio Serena, Carrieri Claudia, Morgan Marcos, Vasiliauskaite Lina, Benes Vladimir, Enright Anton J, O'Carroll Dónal, ;2013;Molecular cell;50;601-8; 23706823
- G9a co-suppresses LINE1 elements in spermatogonia.;Di Giacomo Monica, Comazzetto Stefano, Sampath Srihari C, Sampath Srinath C, O'Carroll Dónal, ;2014;Epigenetics & chromatin;7;24; 25276231
- Piwil2 (Mili) sustains neurogenesis and prevents cellular senescence in the postnatal hippocampus.;Gasperini Caterina, Tuntevski Kiril, Beatini Silvia, Pelizzoli Roberta, Lo Van Amanda, Mangoni Damiano, Cossu Rosa M, Pascarella Giovanni, Bianchini Paolo, Bielefeld Pascal, Scarpato Margherita, Pons-Espinal Meritxell, Sanges Remo, Diaspro Alberto, Fitzsimons Carlos P, Carninci Piero, Gustincich Stefano, De Pietri Tonelli Davide, ;2023;EMBO reports;24;e53801; 36472244