C57BL/6N-Fbxo11^{tm2a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:06999
International strain name	C57BL/6N-Fbxo11^{tm2a(EUCOMM)Wtsi}/H
Alternative name	EPD0248_1_H03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Fbxo11^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Fbxo11
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0248_1_H03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased prepulse inhibition / IMPC
increased lean body mass / IMPC
decreased hematocrit / IMPC
thrombocytopenia / IMPC
decreased circulating iron level / IMPC
decreased heart weight / IMPC
increased total body fat amount / IMPC
abnormal startle reflex / IMPC
abnormal defecation / IMPC
abnormal locomotor activation / IMPC
increased startle reflex / IMPC
hyperactivity / IMPC
abnormal vocalization / IMPC
decreased hemoglobin content / IMPC
narrow eye opening / IMPC
preweaning lethality, complete penetrance / IMPC
decreased mean corpuscular volume / IMPC
increased bone mineral content / IMPC
decreased circulating calcium level / IMPC
decreased lean body mass / IMPC
decreased circulating triglyceride level / IMPC
decreased circulating total protein level / IMPC
decreased erythrocyte cell number / IMPC
short tibia / IMPC
abnormal retina morphology / IMPC
increased fasting circulating glucose level / IMPC
increased red blood cell distribution width / IMPC
decreased bone mineral density / IMPC
abnormal bone mineralization / IMPC

MGI phenotypes (allele matching)

decreased hair follicle number / MGI
thick epidermis / MGI
epidermal hyperplasia / MGI
abnormal protein level / MGI
absent keratohyalin granules / MGI
neonatal lethality, complete penetrance / MGI

MGI phenotypes (gene matching)

midline facial cleft / MGI
cleft palate / MGI
decreased hair follicle number / MGI
short snout / MGI
thick epidermis / MGI
epidermal hyperplasia / MGI
decreased body weight / MGI
decreased body size / MGI
eyelids open at birth / MGI
abnormal pinna reflex / MGI
decreased startle reflex / MGI
increased susceptibility to otitis media / MGI
deafness / MGI
abnormal cytokine secretion / MGI
abnormal auditory tube / MGI
decreased endocochlear potential / MGI
abnormal middle ear ossicle morphology / MGI
hearing/vestibular/ear phenotype / MGI
impaired hearing / MGI
absent pinna reflex / MGI
abnormal protein level / MGI
absent keratohyalin granules / MGI
neonatal lethality, complete penetrance / MGI
neonatal lethality, incomplete penetrance / MGI
perinatal lethality, incomplete penetrance / MGI
short face / MGI
abnormal tympanic cavity morphology / MGI
middle ear polyps / MGI
middle ear effusion / MGI
tympanic membrane retraction / MGI
excessive cerumen / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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