B6Dnk;B6Brd;B6N-Tyr^c-Brd Pfn1^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:06990
Citation information	RRID:IMSR_EM:06990 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Dnk;B6Brd;B6N-Tyr^c-Brd Pfn1^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0046_1_C10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Pfn1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Pfn1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0046_1_C10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Amyotrophic lateral sclerosis / Orphanet_803

IMPC phenotypes (allele matching)

decreased body weight / IMPC
fused cornea and lens / IMPC
abnormal cornea morphology / IMPC
corneal opacity / IMPC
abnormal coat/hair pigmentation / IMPC
decreased lean body mass / IMPC
improved glucose tolerance / IMPC
increased circulating amylase level / IMPC
decreased total body fat amount / IMPC
decreased circulating fructosamine level / IMPC
increased circulating magnesium level / IMPC
decreased bone mineral content / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal cornea morphology / IMPC
fused cornea and lens / IMPC
increased circulating amylase level / IMPC
improved glucose tolerance / IMPC
decreased bone mineral content / IMPC
corneal opacity / IMPC
abnormal coat/hair pigmentation / IMPC
decreased body weight / IMPC
increased circulating magnesium level / IMPC
decreased circulating fructosamine level / IMPC
decreased total body fat amount / IMPC
preweaning lethality, complete penetrance / IMPC
decreased lean body mass / IMPC

MGI phenotypes (allele matching)

decreased body weight / MGI
decreased total body fat amount / MGI
decreased circulating fructosamine level / MGI
decreased bone mineral content / MGI

MGI phenotypes (gene matching)

decreased body weight / MGI
no abnormal phenotype detected / MGI
decreased total body fat amount / MGI
decreased circulating fructosamine level / MGI
decreased bone mineral content / MGI
perinatal lethality, incomplete penetrance / MGI
embryonic lethality before implantation, complete penetrance / MGI
prenatal lethality, incomplete penetrance / MGI
lethality, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Dnk;B6Brd;B6N-Tyrc-Brd Pfn1tm1a(EUCOMM)Wtsi/WtsiBiat