B6;CBA-Tg(TNF)3647Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:06971 |
| Citation information | RRID:IMSR_EM:06971 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(TNF)3647Gkl/Flmg |
| Alternative name | B6;CBA-Tg(TNF-HBB)3647Gkl/Flmg, B6;CBA-Tg(huTNF)3647Gkl/Flmg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(TNF)3647Gkl |
| Gene/Transgene symbol | Tg(TNF)3647Gkl |
Information from provider
| Provider | George KOLLIAS |
| Provider affiliation | Institute of Immunology, BSRC Alexander Fleming |
| Genetic information | The human TNF/beta-globin (TNF-globin) recombinant gene construct was prepared in the pBluescript vector as follows: The 2.8 kb EcoRI fragment which contains the entire coding region of the human TNF gene was ligated to the 0.77 kb EcoRI-Sall fragment which contains the 3'-untranslated region and polyadenylation site of the human beta-globin gene (Kollias et al., 1987) and excised for microinjection as a BamHI-Sall fragment. |
| Phenotypic information | Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis. In contrast to the other line expressing the same transgene (Tg(TNF)197Gkl), the onset of arthritis occurs later on in adult life. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous mixed C57BL/6 x CBA males, wild-type (C57BL/6 x CBA)F1 females |
| Stage of embryos | Morula |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.;Keffer J, Probert L, Cazlaris H, Georgopoulos S, Kaslaris E, Kioussis D, Kollias G, ;1991;The EMBO journal;10;4025-31; 1721867
- Dendritic cell-specific transmembrane protein is required for synovitis and bone resorption in inflammatory arthritis.;Garcia-Hernandez Maria de la Luz, Rangel-Moreno Javier, Garcia-Castaneda Maricela, Kenney H Mark, Paine Ananta, Thullen Michael, Anandarajah Allen P, Schwarz Edward M, Dirksen Robert T, Ritchlin Christopher T, ;2022;Frontiers in immunology;13;1026574; 36420272
- Single-cell transcriptomics of popliteal lymphatic vessels and peripheral veins reveals altered lymphatic muscle and immune cell populations in the TNF-Tg arthritis model.;Kenney H Mark, Wu Chia-Lung, Loiselle Alayna E, Xing Lianping, Ritchlin Christopher T, Schwarz Edward M, ;2022;Arthritis research & therapy;24;64; 35255954
- Osteoclast-Derived Autotaxin, a Distinguishing Factor for Inflammatory Bone Loss.;Flammier Sacha, Peyruchaud Olivier, Bourguillault Fanny, Duboeuf François, Davignon Jean-Luc, Norman Derek D, Isaac Sylvie, Marotte Hubert, Tigyi Gabor, Machuca-Gayet Irma, Coury Fabienne, ;2019;Arthritis & rheumatology (Hoboken, N.J.);71;1801-1811; 31162832
- Tumor Necrosis Factor Alpha Overexpression Induces Mainly Osteoclastogenesis at the Vertebral Site.;Courbon Guillaume, Flammier Sacha, Laroche Norbert, Vico Laurence, Marotte Hubert, Coury Fabienne, ;2017;Calcified tissue international;100;575-584; 28289800