B6;CBA-Tg(TNF)3647Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:06971 |
| International strain name | B6;CBA-Tg(TNF)3647Gkl/Flmg |
| Alternative name | B6;CBA-Tg(TNF-HBB)3647Gkl/Flmg, B6;CBA-Tg(huTNF)3647Gkl/Flmg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(TNF)3647Gkl |
| Gene/Transgene symbol | Tg(TNF)3647Gkl |
Information from provider
| Provider | George KOLLIAS |
| Provider affiliation | Institute of Immunology, BSRC Alexander Fleming |
| Genetic information | The human TNF/beta-globin (TNF-globin) recombinant gene construct was prepared in the pBluescript vector as follows: The 2.8 kb EcoRI fragment which contains the entire coding region of the human TNF gene was ligated to the 0.77 kb EcoRI-Sall fragment which contains the 3'-untranslated region and polyadenylation site of the human beta-globin gene (Kollias et al., 1987) and excised for microinjection as a BamHI-Sall fragment. |
| Phenotypic information | Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis. In contrast to the other line expressing the same transgene (Tg(TNF)197Gkl), the onset of arthritis occurs later on in adult life. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous mixed C57BL/6 x CBA, wild-type (C57BL/6 x CBA)F1 |
| Stage of embryos | Morula |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis.;Keffer J, Probert L, Cazlaris H, Georgopoulos S, Kaslaris E, Kioussis D, Kollias G, ;1991;The EMBO journal;10;4025-31; 1721867
- Dendritic cell-specific transmembrane protein is required for synovitis and bone resorption in inflammatory arthritis.;Garcia-Hernandez Maria de la Luz, Rangel-Moreno Javier, Garcia-Castaneda Maricela, Kenney H Mark, Paine Ananta, Thullen Michael, Anandarajah Allen P, Schwarz Edward M, Dirksen Robert T, Ritchlin Christopher T, ;2022;Frontiers in immunology;13;1026574; 36420272
- Single-cell transcriptomics of popliteal lymphatic vessels and peripheral veins reveals altered lymphatic muscle and immune cell populations in the TNF-Tg arthritis model.;Kenney H Mark, Wu Chia-Lung, Loiselle Alayna E, Xing Lianping, Ritchlin Christopher T, Schwarz Edward M, ;2022;Arthritis research & therapy;24;64; 35255954
- Osteoclast-Derived Autotaxin, a Distinguishing Factor for Inflammatory Bone Loss.;Flammier Sacha, Peyruchaud Olivier, Bourguillault Fanny, Duboeuf François, Davignon Jean-Luc, Norman Derek D, Isaac Sylvie, Marotte Hubert, Tigyi Gabor, Machuca-Gayet Irma, Coury Fabienne, ;2019;Arthritis & rheumatology (Hoboken, N.J.);71;1801-1811; 31162832
- Tumor Necrosis Factor Alpha Overexpression Induces Mainly Osteoclastogenesis at the Vertebral Site.;Courbon Guillaume, Flammier Sacha, Laroche Norbert, Vico Laurence, Marotte Hubert, Coury Fabienne, ;2017;Calcified tissue international;100;575-584; 28289800