| Status | Available to order |
| EMMA ID | EM:06943 |
| Citation information | RRID:IMSR_EM:06943 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Rag2tm1Fwa B2mtm1Unc Prf1tm1Sdz H2-Ab1b-tm1Doi Tg(HLA-A/H2-D/B2M)1Bpe Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma/Orl |
| Alternative name | SURE-L1/Rag2°/° Prf°/° |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Prf1tm1Sdz, B2mtm1Unc, H2-Ab1b-tm1Doi, Tg(HLA-A/H2-D/B2M)1Bpe, Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma, Rag2tm1Fwa |
| Gene/Transgene symbol | Prf1, B2m, H2-Ab1, Tg(HLA-A/H2-D/B2M)1Bpe, Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma, Rag2 |
| Provider | Yu Chun LONE |
| Provider affiliation | INSERM U1014 |
| Genetic information | This line is a combination of the previously cryopreserved line, Sure-L1 (EMMA strain ID EM:01783), with additional genes, Rag2 and perforin 1, knocked out. The literature describes two comparable immunodeficient models that may be used for transplant recipient studies, Rag2-/- GammaC-/- and Rag2-/- Prf-/-. The first requires very early transfer of stem cells in newborns to overcome ganglion atrophy that is associated with this model. Rag2-/- GammaC-/- mice also show increased transplant acceptance frequency, as compared to Rag2-/- Prf-/- mice. However, for ease of use and taking advantage of their normal ganglion structures, Rag2-/- Prf-/- provide a good model for transplantation studies, as stem cells can be transferred in adult. |
| Phenotypic information | Only checked by total genotyping and partial phenotyping for the absence of both T and B cells. |
| Breeding history | We transferred mice in heterozygous status to CDTA-EMMA Orleans, where we continue breeding until obtaining homozygous mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | yes |
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males, homozygous C57BL/6J females |
| Breeding at archiving centre | Males are Rag2 homo, beta2m homo, HLAA2 Tg, Perf homo. Females are Rag2 homo, beta2m homo, HLAA2 Tg, Perf homo; or heterozygous for beta2m and Perf. In some cases the females are wild-type for the beta2m and Perf genes. |
| Stage of embryos | 2-cell |
Orphanet associated rare diseases, based on orthologous gene matching
