| Status | Available to order |
| EMMA ID | EM:06943 |
| International strain name | STOCK Rag2tm1Fwa B2mtm1Unc Prf1tm1Sdz H2-Ab1b-tm1Doi Tg(HLA-A/H2-D/B2M)1Bpe Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma/Orl |
| Alternative name | SURE-L1/Rag2°/° Prf°/° |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Prf1tm1Sdz, B2mtm1Unc, H2-Ab1b-tm1Doi, Tg(HLA-A/H2-D/B2M)1Bpe, Tg(HLA-DRA*0101, HLA-DRB1*0101)1Dma |
| Gene/Transgene symbol | Prf1, B2m, H2-Ab1, Tg(HLA-A/H2-D/B2M)1Bpe, Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma, Rag2 |
| Provider | Yu Chun LONE |
| Provider affiliation | INSERM U1014 |
| Genetic information | This line is a combination of the previously cryopreserved line, Sure-L1 (EM:01783), with additional genes, Rag2 and perforin knocked out. The literature describes two comparable immunodeficient models that may be used for transplant recipient studies, Rag2-/- GammaC-/- and Rag2-/- Prf-/-. The first requires very early transfer of stem cells in newborns to overcome ganglion atrophy that is associated with this model. Rag2-/- GammaC-/- mice also show increased transplant acceptance frequency, as compared to Rag2-/- Prf-/- mice. However, for ease of use and taking advantage of their normal ganglion structures, Rag2-/- Prf-/- provide a good model for transplantation studies, as stem cells can be transferred in adult. |
| Phenotypic information | Only checked by total genotyping and partial phenotyping for the absence of both T and B cells. |
| Breeding history | We transferred mice in heterozygous status to CDTA-EMMA Orleans, where we continue breeding until obtaining homozygous mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | yes |
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J, homozygous C57BL/6J |
| Breeding at archiving centre | Males are Rag2, B2m (beta2m), Prf1 (Perf) homozygous knock-out and Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma (HLAA2) transgenic. Females are Rag2, B2m, Prf1 homozygous knock-out and Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma (HLAA2) transgenic, or B2m and Prf1 heterozygous knock-out. In some cases females are wild-type for the B2m and Prf1 genes. |
| Stage of embryos | 2-cell |
Orphanet associated rare diseases, based on orthologous gene matching
