C57BL/6-Tg(UBC-GFP,-Fkbp11)HPsou/Orl
| Status | Available to order |
| EMMA ID | EM:06914 |
| International strain name | C57BL/6-Tg(UBC-GFP,-Fkbp11)HPsou/Orl |
| Alternative name | FKBPhigh |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(UBC-GFP,-Fkbp11)HPsou |
| Gene/Transgene symbol | Tg(UBC-GFP,-Fkbp11)HPsou |
Information from provider
| Provider | Pauline Soulas-Sprauel |
| Provider affiliation | CRBS (Centre de Recherche en Biomédecine), Inserm UMR 1109 (Immuno-Rhumatologie Moléculaire, IRM) |
| Genetic information | This FKBP11high lentiviral transgenic mouse line carries one copy of stable lentiviral integration with GFP reporter. GFP and FKBP19 (protein encoded by Fkbp11 gene) are coexpressed in a stoichiometric manner thanks to a sequence encoding a T2A peptide (derived from picornavirus) between GFP and Fkbp11 sequences. |
| Phenotypic information | FKBP11 mRNA is 7 times over expressed in bone marrow, 36 times in lymph nodes, 8 times in spleen, and 6 times in purified splenic B cells. Normal development. Splenic hyperplasia concerning all lymphoid subpopulations, especially B cells. Increased B cell response to T-dependent antigen in vivo. Increased production of autoantibodies (IgG antidsDNA for example). IgG3 hypergammaglobulinemia. Increased plasma cell differentiation. We have referenced a FKBP11 LOW lentigenic mouse line in EMMA program, with a smaller over expression of FKBP11: the phenotype of this LOW line is very light compared to this "high" line: no hypergammaglobulinemia, no splenic hyperplasia and no autoantibody production in the LOW line. |
| Breeding history | The lentiviral transgenic strain was produced on C57BL/6 background and maintained in the same background by backcrosses. We have selected a one-copy founder which has been studied on different generations and the line is stable (good Mendelian transmission and reproducible results on several generations). |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J |
Literature references
- Overexpression of Fkbp11, a feature of lupus B cells, leads to B cell tolerance breakdown and initiates plasma cell differentiation.;Ruer-Laventie Julie, Simoni Léa, Schickel Jean-Nicolas, Soley Anne, Duval Monique, Knapp Anne-Marie, Marcellin Luc, Lamon Delphine, Korganow Anne-Sophie, Martin Thierry, Pasquali Jean-Louis, Soulas-Sprauel Pauline, ;2015;Immunity, inflammation and disease;3;265-79; 26417441