B6NTac;B6N-A^tm1Brd Cyfip1^{tm2a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:06868
International strain name	B6NTac;B6N-A^tm1Brd Cyfip1^{tm2a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0555_2_B11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cyfip1^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cyfip1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0555_2_B11. For further details on the construction of this clone see the page at the IKMC portal. Removal of the targeting cassette using Flp recombinase is required to convert the targeted into a conditional allele - more information on conversion to the b,c and d allele forms. Click here for more information on EUCOMM final vectors
Phenotypic information	Potential phenotyping data in the IMPC portal
Breeding history	Current information may be viewed at the ^{a href="http://www.sanger.ac.uk/mouseportal/search?query=Cyfip1"}Sanger mouse portal^/a: Viability at weaning, Fertility, General Observations
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6N Tac
Breeding at archiving centre	Frozen material received from depositor for distribution

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased circulating HDL cholesterol level / IMPC

IMPC phenotypes (gene matching)

decreased circulating HDL cholesterol level / IMPC

MGI phenotypes (gene matching)

open neural tube / MGI
abnormal anxiety-related response / MGI
hypoactivity / MGI
abnormal long term depression / MGI
abnormal CNS synaptic transmission / MGI
enhanced paired-pulse facilitation / MGI
kinked neural tube / MGI
delayed somite formation / MGI
abnormal rostral-caudal body axis extension / MGI
enhanced cued conditioning behavior / MGI
impaired somite development / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between somite formation and embryo turning, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6NTac;B6N-Atm1Brd Cyfip1tm2a(EUCOMM)Wtsi/WtsiH