C57BL/6NTac-Setd1a^{tm1a(EUCOMM)Wtsi}/WtsiCnrm

Status	Available to order
EMMA ID	EM:06857
International strain name	C57BL/6NTac-Setd1a^{tm1a(EUCOMM)Wtsi}/WtsiCnrm
Alternative name	EPD0027_4_H10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Setd1a^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Setd1a
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0027_4_H10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (allele matching)

increased bone mineral density / IMPC
decreased circulating LDL cholesterol level / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased leukocyte cell number / IMPC
decreased circulating cholesterol level / IMPC
increased circulating creatinine level / IMPC
increased blood urea nitrogen level / IMPC
decreased total body fat amount / IMPC
increased circulating fructosamine level / IMPC
hyperplasia / IMPC
neoplasm / IMPC
lymphoid neoplasms / IMPC

IMPC phenotypes (gene matching)

lymphoid neoplasms / IMPC
hyperplasia / IMPC
increased blood urea nitrogen level / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased leukocyte cell number / IMPC
decreased circulating LDL cholesterol level / IMPC
increased circulating fructosamine level / IMPC
decreased circulating cholesterol level / IMPC
increased circulating creatinine level / IMPC
decreased total body fat amount / IMPC
increased bone mineral density / IMPC
neoplasm / IMPC

MGI phenotypes (allele matching)

increased bone mineral density / MGI
decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
decreased leukocyte cell number / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating alkaline phosphatase level / MGI
decreased rib number / MGI
decreased circulating cholesterol level / MGI
decreased total body fat amount / MGI

MGI phenotypes (gene matching)

increased bone mineral density / MGI
decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
decreased leukocyte cell number / MGI
failure to gastrulate / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating alkaline phosphatase level / MGI
decreased rib number / MGI
decreased circulating cholesterol level / MGI
cellular phenotype / MGI
abnormal cell physiology / MGI
abnormal DNA methylation / MGI
decreased total body fat amount / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Setd1atm1a(EUCOMM)Wtsi/WtsiCnrm