B6;SJL-Tg(Krt1-15-cre/PGR*)22Cot/Cnbc

Status

Available to order

EMMA IDEM:06791
International strain nameB6;SJL-Tg(Krt1-15-cre/PGR*)22Cot/Cnbc
Alternative nameB6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Krt1-15-cre/PGR*)22Cot
Gene/Transgene symbolTg(Krt1-15-cre/PGR*)22Cot

Information from provider

ProviderJesus Espada
Provider affiliationDepartamento de Biologia del Cancer, Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM
Additional ownerDr. George Cotsarelis, University of Pennsylvania, Dermatology Department, Philadelphia PA, USA
Genetic informationThe transgene comprises the entire 5.0-kb mouse Krt1-15 promoter region driving expression of a fusion protein composed of an amino-terminal nuclear localization signal followed by cre recombinase joined to the carboxyl-terminal hormone binding domain (HBD) of a mutant human progesterone receptor (PGR). This mutant HBD is activated by RU486.
Phenotypic informationConstitutive expression of the Cre fusion protein is directed by the Krt1-15 promoter to epithelial stem cells in the bulge region of the hair follicle. It remains inactive in the cytoplasm until bound to RU486, whereupon it translocates to the nucleus and can catalyze loxP-mediated recombination.
Breeding historyA transgenic construct containing sequence encoding cre/PGR under the control of the mouse keratin complex 1, acidic, gene 15 promoter, was introduced into B6SJLF1 donor eggs and thereafter the resulting founder has been backcrossed to C57BL/6J mice.
References
  • Regulation of Cre recombinase activity by the synthetic steroid RU 486.;Kellendonk C, Tronche F, Monaghan A P, Angrand P O, Stewart F, Schütz G, ;1996;Nucleic acids research;24;1404-11; 8628671
  • Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.;Liu Yaping, Lyle Stephen, Yang Zaixin, Cotsarelis George, ;2003;The Journal of investigative dermatology;121;963-8; 14708593
  • Capturing and profiling adult hair follicle stem cells.;Morris Rebecca J, Liu Yaping, Marles Lee, Yang Zaixin, Trempus Carol, Li Shulan, Lin Jamie S, Sawicki Janet A, Cotsarelis George, ;2004;Nature biotechnology;22;411-7; 15024388
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Literature references

  • Regulation of Cre recombinase activity by the synthetic steroid RU 486.;Kellendonk C, Tronche F, Monaghan A P, Angrand P O, Stewart F, Schütz G, ;1996;Nucleic acids research;24;1404-11; 8628671
  • Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.;Liu Yaping, Lyle Stephen, Yang Zaixin, Cotsarelis George, ;2003;The Journal of investigative dermatology;121;963-8; 14708593
  • Capturing and profiling adult hair follicle stem cells.;Morris Rebecca J, Liu Yaping, Marles Lee, Yang Zaixin, Trempus Carol, Li Shulan, Lin Jamie S, Sawicki Janet A, Cotsarelis George, ;2004;Nature biotechnology;22;411-7; 15024388

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
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EMMA conditions
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