B6;SJL-Tg(Krt1-15-cre/PGR*)22Cot/Cnbc
Status | Available to order |
EMMA ID | EM:06791 |
International strain name | B6;SJL-Tg(Krt1-15-cre/PGR*)22Cot/Cnbc |
Alternative name | B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(Krt1-15-cre/PGR*)22Cot |
Gene/Transgene symbol | Tg(Krt1-15-cre/PGR*)22Cot |
Information from provider
Provider | Jesus Espada |
Provider affiliation | Departamento de Biologia del Cancer, Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM |
Additional owner | Dr. George Cotsarelis, University of Pennsylvania, Dermatology Department, Philadelphia PA, USA |
Genetic information | The transgene comprises the entire 5.0-kb mouse Krt1-15 promoter region driving expression of a fusion protein composed of an amino-terminal nuclear localization signal followed by cre recombinase joined to the carboxyl-terminal hormone binding domain (HBD) of a mutant human progesterone receptor (PGR). This mutant HBD is activated by RU486. |
Phenotypic information | Constitutive expression of the Cre fusion protein is directed by the Krt1-15 promoter to epithelial stem cells in the bulge region of the hair follicle. It remains inactive in the cytoplasm until bound to RU486, whereupon it translocates to the nucleus and can catalyze loxP-mediated recombination. |
Breeding history | A transgenic construct containing sequence encoding cre/PGR under the control of the mouse keratin complex 1, acidic, gene 15 promoter, was introduced into B6SJLF1 donor eggs and thereafter the resulting founder has been backcrossed to C57BL/6J mice. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Literature references
- Regulation of Cre recombinase activity by the synthetic steroid RU 486.;Kellendonk C, Tronche F, Monaghan A P, Angrand P O, Stewart F, Schütz G, ;1996;Nucleic acids research;24;1404-11; 8628671
- Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.;Liu Yaping, Lyle Stephen, Yang Zaixin, Cotsarelis George, ;2003;The Journal of investigative dermatology;121;963-8; 14708593
- Capturing and profiling adult hair follicle stem cells.;Morris Rebecca J, Liu Yaping, Marles Lee, Yang Zaixin, Trempus Carol, Li Shulan, Lin Jamie S, Sawicki Janet A, Cotsarelis George, ;2004;Nature biotechnology;22;411-7; 15024388
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