STOCK Sirt3^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:06779
International strain name	STOCK Sirt3^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0496_2_F10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sirt3^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Sirt3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0496_2_F10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac
Breeding at archiving centre	Chimeras were mated to C57BL/6NTac and maintained on this genetic background. Due to the use of the C57BL/6N-A^tm1Brd/a ES cells, agouti and non-agouti offspring may be generated.

Disease and phenotype information

IMPC phenotypes (gene matching)

increased circulating alanine transaminase level / IMPC
increased circulating creatine kinase level / IMPC
abnormal gait / IMPC
vertebral fusion / IMPC

MGI phenotypes (gene matching)

increased circulating LDL cholesterol level / MGI
increased circulating triglyceride level / MGI
hyperglycemia / MGI
liver inflammation / MGI
increased circulating insulin level / MGI
abnormal lipid homeostasis / MGI
no abnormal phenotype detected / MGI
hepatic steatosis / MGI
no phenotypic analysis / MGI
liver fibrosis / MGI
increased circulating VLDL cholesterol level / MGI
increased circulating cholesterol level / MGI
decreased oxygen consumption / MGI
abnormal enzyme/coenzyme level / MGI
insulin resistance / MGI
abnormal cell physiology / MGI
increased susceptibility to diet-induced obesity / MGI
increased systemic arterial systolic blood pressure / MGI
increased circulating tumor necrosis factor level / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-10 level / MGI
increased circulating interleukin-6 level / MGI
increased circulating interleukin-12 level / MGI
abnormal cytokine level / MGI
decreased carbon dioxide production / MGI
increased liver triglyceride level / MGI
increased total body fat amount / MGI
increased liver cholesterol level / MGI
mortality/aging / MGI
abnormal cellular respiration / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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