B6.129P2-Irf4^tm1Mak/Cnbc

Status	Available to order
EMMA ID	EM:06742
International strain name	B6.129P2-Irf4^tm1Mak/Cnbc
Alternative name	Irf4
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Irf4^tm1Mak
Gene/Transgene symbol	Irf4

Information from provider

Provider	Tak W Mak
Provider affiliation	Mak Lab, Campbell Family Breast Cancer reseach Institute
Genetic information	Exons 2 and 3 replaced with neomycin resistance gene.
Phenotypic information	Irf4 deficient mice develop progressive Lymphadenopathy, reduction in serum immunoglobulin concentrations, do not mount detectable antibody response, T lymphocyte function impaired.
Breeding history	Backcrossed to C57BL/6 10 times. Bred as heterozygous.
References	Requirement for the transcription factor LSIRF/IRF4 for mature B and T lymphocyte function.;Mittrücker H W, Matsuyama T, Grossman A, Kündig T M, Potter J, Shahinian A, Wakeham A, Patterson B, Ohashi P S, Mak T W, ;1997;Science (New York, N.Y.);275;540-3; 8999800 Transcription factor IRF4 determines germinal center formation through follicular T-helper cell differentiation.;Bollig Nadine, Brüstle Anne, Kellner Kerstin, Ackermann Waltraud, Abass Elfadil, Raifer Hartmann, Camara Bärbel, Brendel Cornelia, Giel Gavin, Bothur Evita, Huber Magdalena, Paul Christoph, Elli Alexandra, Kroczek Richard A, Nurieva Roza, Dong Chen, Jacob Ralf, Mak Tak W, Lohoff Michael, ;2012;Proceedings of the National Academy of Sciences of the United States of America;109;8664-9; 22552227
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	yes

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Whipple disease / Orphanet_3452

IMPC phenotypes (gene matching)

decreased lymphocyte cell number / IMPC
enlarged spleen / IMPC
abnormal spleen morphology / IMPC
increased spleen weight / IMPC
abnormal startle reflex / IMPC
increased neutrophil cell number / IMPC
enlarged lymph nodes / IMPC
abnormal lymph node morphology / IMPC
abnormal thymus morphology / IMPC
enlarged thymus / IMPC

MGI phenotypes (allele matching)

increased susceptibility to bacterial infection / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
abnormal T-helper 1 cell differentiation / MGI
abnormal spleen morphology / MGI
enlarged spleen / MGI
enlarged lymph nodes / MGI
abnormal immune system cell morphology / MGI
abnormal immune system physiology / MGI
arrested B cell differentiation / MGI
abnormal B cell differentiation / MGI
altered tumor susceptibility / MGI
abnormal lymph node morphology / MGI
increased susceptibility to viral infection / MGI
decreased immunoglobulin level / MGI
increased lymphocyte cell number / MGI
decreased B cell proliferation / MGI
decreased T cell proliferation / MGI
immune system phenotype / MGI
absent plasma cells / MGI
decreased dendritic cell number / MGI
absent spleen germinal center / MGI
absent lymph node germinal center / MGI
decreased plasmacytoid dendritic cell number / MGI

MGI phenotypes (gene matching)

abnormal spleen morphology / MGI
enlarged spleen / MGI
enlarged lymph nodes / MGI
abnormal immune system cell morphology / MGI
abnormal immune system physiology / MGI
arrested B cell differentiation / MGI
decreased IgG level / MGI
decreased IgM level / MGI
abnormal B cell differentiation / MGI
altered tumor susceptibility / MGI
abnormal lymph node morphology / MGI
increased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
abnormal B cell physiology / MGI
decreased immunoglobulin level / MGI
abnormal class switch recombination / MGI
increased lymphocyte cell number / MGI
decreased B cell proliferation / MGI
decreased T cell proliferation / MGI
immune system phenotype / MGI
abnormal T-helper 1 cell differentiation / MGI
absent plasma cells / MGI
decreased plasmacytoid dendritic cell number / MGI
decreased dendritic cell number / MGI
abnormal B cell activation / MGI
absent spleen germinal center / MGI
decreased IgG1 level / MGI
absent lymph node germinal center / MGI

Literature references

Requirement for the transcription factor LSIRF/IRF4 for mature B and T lymphocyte function.;Mittrücker H W, Matsuyama T, Grossman A, Kündig T M, Potter J, Shahinian A, Wakeham A, Patterson B, Ohashi P S, Mak T W, ;1997;Science (New York, N.Y.);275;540-3; 8999800
Transcription factor IRF4 determines germinal center formation through follicular T-helper cell differentiation.;Bollig Nadine, Brüstle Anne, Kellner Kerstin, Ackermann Waltraud, Abass Elfadil, Raifer Hartmann, Camara Bärbel, Brendel Cornelia, Giel Gavin, Bothur Evita, Huber Magdalena, Paul Christoph, Elli Alexandra, Kroczek Richard A, Nurieva Roza, Dong Chen, Jacob Ralf, Mak Tak W, Lohoff Michael, ;2012;Proceedings of the National Academy of Sciences of the United States of America;109;8664-9; 22552227

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Irf4tm1Mak/Cnbc