MGI phenotypes (allele matching)
B6.129P2-Rhoctm1Mak/Cnbc
| Status | Available to order |
| EMMA ID | EM:06741 |
| Citation information | RRID:IMSR_EM:06741 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Rhoctm1Mak/Cnbc |
| Alternative name | RhoC |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rhoctm1Mak |
| Gene/Transgene symbol | Rhoc |
Information from provider
| Provider | Tak W Mak |
| Provider affiliation | Mak Lab, Campbell Family Breast Cancer reseach Institute |
| Genetic information | Exons 2 and 3 flanked with loxP and ES clones transfected with cre recombinase. ES clones with deleted exons 2 and 3 where injected to produce the RhoC knock-out. Deletion of RhoC exons 2 and 3 should introduce termination codons into all three reading frames and remove 70% of the protein sequence. |
| Phenotypic information | RhoC+/- mice were then intercrossed to obtain homozygous RhoCDelta2-3/Delta2-3 (RhoC-/-) mice. RhoC-/- mice were born at the expected Mendelian ratio and were viable and fertile. RhoC is essential for metastasis but not for primary tumor formation. |
| Breeding history | Backcross to C57BL/6. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
Literature references
- RhoC is dispensable for embryogenesis and tumor initiation but essential for metastasis.;Hakem Anne, Sanchez-Sweatman Otto, You-Ten Annick, Duncan Gordon, Wakeham Andrew, Khokha Rama, Mak Tak W, ;2005;Genes & development;19;1974-9; 16107613