MGI phenotypes (allele matching)
B6.129P2-Rhoctm1Mak/Cnbc
| Status | Available to order |
| EMMA ID | EM:06741 |
| International strain name | B6.129P2-Rhoctm1Mak/Cnbc |
| Alternative name | RhoC |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rhoctm1Mak |
| Gene/Transgene symbol | Rhoc |
Information from provider
| Provider | Tak W Mak |
| Provider affiliation | Mak Lab, Campbell Family Breast Cancer reseach Institute |
| Genetic information | Exons 2 and 3 flanked with loxP and ES clones transfected with cre recombinase. ES clones with deleted exons 2 and 3 where injected to produce the RhoC knock-out. Deletion of RhoC exons 2 and 3 should introduce termination codons into all three reading frames and remove 70% of the protein sequence. |
| Phenotypic information | RhoC+/- mice were then intercrossed to obtain homozygous RhoCDelta2-3/Delta2-3 (RhoC-/-) mice. RhoC-/- mice were born at the expected Mendelian ratio and were viable and fertile. RhoC is essential for metastasis but not for primary tumor formation. |
| Breeding history | Backcross to C57BL/6. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J |
Disease and phenotype information
Literature references
- RhoC is dispensable for embryogenesis and tumor initiation but essential for metastasis.;Hakem Anne, Sanchez-Sweatman Otto, You-Ten Annick, Duncan Gordon, Wakeham Andrew, Khokha Rama, Mak Tak W, ;2005;Genes & development;19;1974-9; 16107613