B6.129P2-Irak4^tm1Yeh/Cnbc

Status	Available to order
EMMA ID	EM:06739
International strain name	B6.129P2-Irak4^tm1Yeh/Cnbc
Alternative name	Irak 4
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Irak4^tm1Yeh
Gene/Transgene symbol	Irak4

Information from provider

Provider	Tak W Mak
Provider affiliation	Mak Lab, Campbell Family Breast Cancer reseach Institute
Genetic information	We generated Irak-4-/- mice by gene targeting. A clone containing the genomic mouse Irak-4 gene was isolated from a 129/J library and a targeting vector was constructed by replacing exon 2 (containing the start codon) with a neomycin-resistance cassette.
Phenotypic information	Gene targeting of IRAK-4, which is indispensable for the responses of animals and cultured cells to IL-1 and ligands that stimulate various TLRs. IRAK-4 deficient animals are completely resistant to a lethal dose of lipopolysaccharide (LPS). In addition, animals lacking IRAK-4 are severely impaired in their responses to viral and bacterial challenges.
Breeding history	Bred as homozygous, backcross to C57BL/6. More than 10 backcrosses to C57BL/6.
References	Severe impairment of interleukin-1 and Toll-like receptor signalling in mice lacking IRAK-4.;Suzuki Nobutaka, Suzuki Shinobu, Duncan Gordon S, Millar Douglas G, Wada Teiji, Mirtsos Christine, Takada Hidetoshi, Wakeham Andrew, Itie Annick, Li Shyun, Penninger Josef M, Wesche Holger, Ohashi Pamela S, Mak Tak W, Yeh Wen-Chen, ;2002;Nature;416;750-6; 11923871 Epstein-Barr virus latent membrane protein 1 activation of NF-kappaB through IRAK1 and TRAF6.;Luftig Micah, Prinarakis Efthimios, Yasui Teruhito, Tsichritzis Theodore, Cahir-McFarland Ellen, Inoue Jun-Ichiro, Nakano Hiroyasu, Mak Tak Wah, Yeh Wen-Chen, Li Xiaoxia, Akira Shizuo, Suzuki Nobutaka, Suzuki Shinobu, Mosialos George, Kieff Elliott, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;15595-600; 14673102
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Immunodeficiency due to interleukin-1 receptor-associated kinase-4 deficiency / Orphanet_70592

MGI phenotypes (allele matching)

increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
abnormal cytokine secretion / MGI
decreased B cell proliferation / MGI
decreased circulating tumor necrosis factor level / MGI
decreased tumor necrosis factor secretion / MGI
decreased circulating interferon-gamma level / MGI
decreased circulating interleukin-1 level / MGI
decreased circulating interleukin-6 level / MGI
decreased interleukin-6 secretion / MGI
abnormal cytokine level / MGI
decreased susceptibility to endotoxin shock / MGI
abnormal NK cell physiology / MGI

MGI phenotypes (gene matching)

increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
abnormal cytokine secretion / MGI
decreased acute inflammation / MGI
decreased B cell proliferation / MGI
immune system phenotype / MGI
abnormal cell physiology / MGI
decreased circulating tumor necrosis factor level / MGI
decreased tumor necrosis factor secretion / MGI
decreased interferon-alpha secretion / MGI
decreased interferon-beta secretion / MGI
decreased circulating interferon-gamma level / MGI
decreased circulating interleukin-1 level / MGI
decreased circulating interleukin-6 level / MGI
decreased interleukin-12b secretion / MGI
decreased interleukin-6 secretion / MGI
abnormal cytokine level / MGI
abnormal chemokine secretion / MGI
decreased susceptibility to endotoxin shock / MGI
abnormal NK cell physiology / MGI

Literature references

Severe impairment of interleukin-1 and Toll-like receptor signalling in mice lacking IRAK-4.;Suzuki Nobutaka, Suzuki Shinobu, Duncan Gordon S, Millar Douglas G, Wada Teiji, Mirtsos Christine, Takada Hidetoshi, Wakeham Andrew, Itie Annick, Li Shyun, Penninger Josef M, Wesche Holger, Ohashi Pamela S, Mak Tak W, Yeh Wen-Chen, ;2002;Nature;416;750-6; 11923871
Epstein-Barr virus latent membrane protein 1 activation of NF-kappaB through IRAK1 and TRAF6.;Luftig Micah, Prinarakis Efthimios, Yasui Teruhito, Tsichritzis Theodore, Cahir-McFarland Ellen, Inoue Jun-Ichiro, Nakano Hiroyasu, Mak Tak Wah, Yeh Wen-Chen, Li Xiaoxia, Akira Shizuo, Suzuki Nobutaka, Suzuki Shinobu, Mosialos George, Kieff Elliott, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;15595-600; 14673102

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Irak4tm1Yeh/Cnbc