C57BL/6NTac-Usp22^{tm1a(KOMP)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:06392
International strain name	C57BL/6NTac-Usp22^{tm1a(KOMP)Wtsi}/WtsiH
Alternative name	EPD0119_2_D11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Usp22^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Usp22
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0119_2_D11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

increased lean body mass / IMPC
increased mean corpuscular hemoglobin / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
increased red blood cell distribution width / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased monocyte cell number / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal gait / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
increased lean body mass / IMPC
increased red blood cell distribution width / IMPC
increased mean corpuscular hemoglobin / IMPC

MGI phenotypes (allele matching)

abnormal intestinal epithelium morphology / MGI
abnormal small intestine morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal stratification in cerebral cortex / MGI
decreased body weight / MGI
postnatal growth retardation / MGI
abnormal cell differentiation / MGI
abnormal cortical plate morphology / MGI
mortality/aging / MGI
abnormal intestinal enteroendocrine cell morphology / MGI
impaired neuron differentiation / MGI
increased Paneth cell number / MGI
abnormal intestinal villus goblet cell morphology / MGI

MGI phenotypes (gene matching)

abnormal intestinal epithelium morphology / MGI
abnormal small intestine morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal stratification in cerebral cortex / MGI
decreased body weight / MGI
decreased embryo size / MGI
postnatal growth retardation / MGI
embryonic growth retardation / MGI
abnormal cell differentiation / MGI
abnormal cortical plate morphology / MGI
mortality/aging / MGI
abnormal intestinal enteroendocrine cell morphology / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
impaired neuron differentiation / MGI
increased Paneth cell number / MGI
increased fibroblast apoptosis / MGI
abnormal intestinal villus goblet cell morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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