B6Brd;B6N-Tyr^c-Brd Lmnb1^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:06376
International strain name	B6Brd;B6N-Tyr^c-Brd Lmnb1^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0070_1_G07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Lmnb1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Lmnb1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0070_1_G07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal dominant primary microcephaly / Orphanet_2514
Adult-onset autosomal dominant leukodystrophy / Orphanet_99027

IMPC phenotypes (allele matching)

decreased body weight / IMPC
lipid deposition / IMPC

IMPC phenotypes (gene matching)

decreased body weight / IMPC
lipid deposition / IMPC

MGI phenotypes (allele matching)

decreased circulating calcium level / MGI
decreased body weight / MGI
decreased circulating alkaline phosphatase level / MGI
increased mean corpuscular hemoglobin / MGI

MGI phenotypes (gene matching)

delayed bone ossification / MGI
scoliosis / MGI
decreased circulating calcium level / MGI
decreased cell proliferation / MGI
microcephaly / MGI
abnormal cranium morphology / MGI
thin diaphragm muscle / MGI
decreased brain size / MGI
abnormal stratification in cerebral cortex / MGI
abnormal lung morphology / MGI
abnormal lung development / MGI
absent pulmonary alveoli / MGI
decreased body weight / MGI
decreased embryo size / MGI
respiratory failure / MGI
abnormal pulmonary alveolus morphology / MGI
abnormal neuron morphology / MGI
decreased circulating alkaline phosphatase level / MGI
abnormal thoracic vertebrae morphology / MGI
abnormal cell nucleus morphology / MGI
abnormal coronal suture morphology / MGI
abnormal sagittal suture morphology / MGI
decreased fetal size / MGI
abnormal neuronal precursor proliferation / MGI
abnormal cell differentiation / MGI
increased mean corpuscular hemoglobin / MGI
abnormal neuronal migration / MGI
impaired lung alveolus development / MGI
neonatal lethality, complete penetrance / MGI
abnormal phrenic nerve innervation pattern to diaphragm / MGI
decreased midbrain size / MGI
flat head / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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