B6.129P2-Casp9tm1Mak/Cnbc

Status

Available to order

EMMA IDEM:06374
International strain nameB6.129P2-Casp9tm1Mak/Cnbc
Alternative nameCaspase 9
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCasp9tm1Mak
Gene/Transgene symbolCasp9

Information from provider

ProviderTak W Mak
Provider affiliationMak Lab, Campbell Family Breast Cancer reseach Institute
Genetic informationA targeting vector was designed to delete 2.6 kb of genomic DNA containing three coding exons of Casp9. This strategy led to the introduction of termination codons into all three reading frames and the removal of 138 amino acids, including the key pentapeptide QACGG, required for Casp9 activity.
Phenotypic informationInterbreeding of Casp9+/- mice showed that homozygosity for the Casp9 mutation was lethal, since fewer than 8% of postnatal day (pnd) 1 mice were homozygotes. At birth, Casp9-/- mice were consistently smaller than control littermates, and most died before pnd 3. Timed matings indicated that the onset of abnormalities was at about E12.5. At E12.5 and E15.5, Casp9-/- mice displayed protrusions of the brain tissue from the skull. These data show that Casp9 is required for embryonic and postnatal survival.
Breeding historyMust be bred as heterozygous. Pure C57BL/6.
References
  • Differential requirement for caspase 9 in apoptotic pathways in vivo.;Hakem R, Hakem A, Duncan G S, Henderson J T, Woo M, Soengas M S, Elia A, de la Pompa J L, Kagi D, Khoo W, Potter J, Yoshida R, Kaufman S A, Lowe S W, Penninger J M, Mak T W, ;1998;Cell;94;339-52; 9708736
  • Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition.;Soengas M S, Alarcón R M, Yoshida H, Giaccia A J, Hakem R, Mak T W, Lowe S W, ;1999;Science (New York, N.Y.);284;156-9; 10102818
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal cerebral cortex morphology / MGI
  • abnormal brain ventricle morphology / MGI
  • abnormal lateral ventricle morphology / MGI
  • decreased body size / MGI
  • hydroencephaly / MGI
  • intracerebral hemorrhage / MGI
  • absent corpus callosum / MGI
  • abnormal brain commissure morphology / MGI
  • decreased neuron apoptosis / MGI
  • decreased cellular sensitivity to gamma-irradiation / MGI
  • abnormal forebrain development / MGI
  • immune system phenotype / MGI
  • abnormal cell physiology / MGI
  • decreased apoptosis / MGI
  • abnormal cerebral hemisphere morphology / MGI
  • decreased sensitivity to induced cell death / MGI
  • abnormal thymocyte apoptosis / MGI
  • abnormal splenocyte apoptosis / MGI
  • decreased birth body size / MGI
  • abnormal respiratory electron transport chain / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
MGI phenotypes (gene matching)
  • abnormal embryonic neuroepithelial layer differentiation / MGI
  • abnormal telencephalon morphology / MGI
  • abnormal cerebral cortex morphology / MGI
  • abnormal brain ventricle morphology / MGI
  • abnormal lateral ventricle morphology / MGI
  • abnormal third ventricle morphology / MGI
  • abnormal brain development / MGI
  • exencephaly / MGI
  • abnormal telencephalon development / MGI
  • decreased body size / MGI
  • abnormal apoptosis / MGI
  • hydroencephaly / MGI
  • intracerebral hemorrhage / MGI
  • perinatal lethality / MGI
  • abnormal brain morphology / MGI
  • absent corpus callosum / MGI
  • abnormal brain commissure morphology / MGI
  • decreased neuron apoptosis / MGI
  • decreased cellular sensitivity to gamma-irradiation / MGI
  • abnormal forebrain development / MGI
  • abnormal neural tube closure / MGI
  • embryo phenotype / MGI
  • cellular phenotype / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • abnormal cell physiology / MGI
  • decreased apoptosis / MGI
  • decreased T cell apoptosis / MGI
  • abnormal cerebral hemisphere morphology / MGI
  • abnormal olfactory epithelium morphology / MGI
  • decreased sensitivity to induced cell death / MGI
  • abnormal thymocyte apoptosis / MGI
  • abnormal splenocyte apoptosis / MGI
  • decreased birth body size / MGI
  • abnormal respiratory electron transport chain / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • increased midbrain size / MGI
  • abnormal midbrain-hindbrain boundary morphology / MGI
  • abnormal lamina terminalis morphology / MGI
  • brain ventricle stenosis / MGI

Literature references

  • Differential requirement for caspase 9 in apoptotic pathways in vivo.;Hakem R, Hakem A, Duncan G S, Henderson J T, Woo M, Soengas M S, Elia A, de la Pompa J L, Kagi D, Khoo W, Potter J, Yoshida R, Kaufman S A, Lowe S W, Penninger J M, Mak T W, ;1998;Cell;94;339-52; 9708736
  • Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition.;Soengas M S, Alarcón R M, Yoshida H, Giaccia A J, Hakem R, Mak T W, Lowe S W, ;1999;Science (New York, N.Y.);284;156-9; 10102818
  • Molecular mechanism of RIPK1 and caspase-8 in homeostatic type I interferon production and regulation.;Wang Yaqiu, Karki Rajendra, Mall Raghvendra, Sharma Bhesh Raj, Kalathur Ravi C, Lee SangJoon, Kancharana Balabhaskararao, So Matthew, Combs Katie L, Kanneganti Thirumala-Devi, ;2022;Cell reports;41;111434; 36198273

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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