MGI phenotypes (allele matching)
B6.129P2-Casp3tm1Mak/Cnbc
| Status | Available to order |
| EMMA ID | EM:06370 |
| International strain name | B6.129P2-Casp3tm1Mak/Cnbc |
| Alternative name | Caspase 3 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Casp3tm1Mak |
| Gene/Transgene symbol | Casp3 |
Information from provider
| Provider | Tak W Mak |
| Provider affiliation | Mak Lab, Campbell Family Breast Cancer reseach Institute |
| Genetic information | The CPP32 (Casp3) gene was disrupted in ES cells by use of a targeting vector that deleted exon 3 of CPP32 gene, leading to the introduction of termination codons in all three reading frames. |
| Phenotypic information | CPP32ex3 mutant mice were born at lower than the expected Mendelian frequency (9%). Most survived until only 4-5 weeks of age. Consistent with the previously reported CPP32 mutant mice, our CPP32ex3-/- mice were smaller than their littermates, showed neurological abnormalities, and had visible masses in their heads that represent ectopic masses of supernumerary cells in place of pyknotic clusters that represent normal apoptosis that occur during brain development. |
| Breeding history | CPP32ex3 mutant mice were born at lower than the expected Mendelian frequency (9%). Most survived until only 4-5 weeks of age. Currently pure C57BL/6. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- cochlear degeneration / MGI
- abnormal organ of Corti morphology / MGI
- delayed bone ossification / MGI
- decreased bone mineral density / MGI
- cleft palate / MGI
- abnormal bone marrow development / MGI
- decreased thymocyte number / MGI
- abnormal forebrain morphology / MGI
- abnormal olfactory bulb morphology / MGI
- abnormal diencephalon morphology / MGI
- abnormal cerebellum development / MGI
- abnormal cerebellum external granule cell layer morphology / MGI
- abnormal cerebellar granule layer morphology / MGI
- abnormal cerebellar molecular layer / MGI
- cranioschisis / MGI
- abnormal ovarian follicle morphology / MGI
- decreased body size / MGI
- abnormal eye development / MGI
- microphthalmia / MGI
- cataract / MGI
- abnormal retina morphology / MGI
- abnormal gait / MGI
- abnormal osteoclast physiology / MGI
- abnormal blood vessel morphology / MGI
- abnormal apoptosis / MGI
- decreased inflammatory response / MGI
- hydroencephaly / MGI
- deafness / MGI
- premature death / MGI
- abnormal eye morphology / MGI
- abnormal ear morphology / MGI
- abnormal skeleton development / MGI
- abnormal brain morphology / MGI
- abnormal cochlear hair cell morphology / MGI
- abnormal olfactory bulb development / MGI
- cochlear ganglion degeneration / MGI
- abnormal bone remodeling / MGI
- no phenotypic analysis / MGI
- decreased neuron apoptosis / MGI
- increased cardiomyocyte apoptosis / MGI
- abnormal lens epithelium morphology / MGI
- abnormal keratinocyte physiology / MGI
- nervous system phenotype / MGI
- abnormal retinal inner nuclear layer morphology / MGI
- abnormal sagittal suture morphology / MGI
- abnormal optic stalk morphology / MGI
- cochlear hair cell degeneration / MGI
- cochlear inner hair cell degeneration / MGI
- cochlear outer hair cell degeneration / MGI
- abnormal outer hair cell stereociliary bundle morphology / MGI
- fused outer hair cell stereocilia / MGI
- absent distortion product otoacoustic emissions / MGI
- abnormal retinal pigment epithelium morphology / MGI
- abnormal brainstem morphology / MGI
- renal/urinary system phenotype / MGI
- hearing/vestibular/ear phenotype / MGI
- cellular phenotype / MGI
- immune system phenotype / MGI
- respiratory system phenotype / MGI
- reproductive system phenotype / MGI
- vision/eye phenotype / MGI
- abnormal lens development / MGI
- decreased apoptosis / MGI
- abnormal olfactory sensory neuron morphology / MGI
- absent pinna reflex / MGI
- absent startle reflex / MGI
- abnormal bone ossification / MGI
- increased cellular sensitivity to ultraviolet irradiation / MGI
- abnormal granulosa cell morphology / MGI
- decreased physiological sensitivity to xenobiotic / MGI
- increased sensitivity to induced cell death / MGI
- decreased sensitivity to induced cell death / MGI
- decreased trabecular bone thickness / MGI
- decreased keratinocyte apoptosis / MGI
- postnatal lethality, complete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- abnormal auditory brainstem response waveform shape / MGI
- increased or absent threshold for auditory brainstem response / MGI
- encephalomeningocele / MGI
- decreased fibroblast apoptosis / MGI
- cranial bossing / MGI
Literature references
- Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes.;Woo M, Hakem R, Soengas M S, Duncan G S, Shahinian A, Kägi D, Hakem A, McCurrach M, Khoo W, Kaufman S A, Senaldi G, Howard T, Lowe S W, Mak T W, ;1998;Genes & development;12;806-19; 9512515
- Caspase 3 is not essential for the induction of anergy or multiple pathways of CD8+ T-cell death.;Murakami Kiichi, Liadis Nicole, Sarmiento Janice, Elford Alisha R, Woo Minna, Nguyen Linh T, Mak Tak W, Ohashi Pamela S, ;2010;European journal of immunology;40;3372-7; 21110320