| Status | Available to order |
| EMMA ID | EM:06302 |
| International strain name | B6.129P2-Ctsbtm1Jde/Ieg |
| Alternative name | Ctsb-ko (Ctsbtm1Jde) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Ctsbtm1Jde |
| Gene/Transgene symbol | Ctsb |
| Provider | Jan Deussing |
| Provider affiliation | Molecular Neurogenetics, Max Planck Institute of Psychiatry |
| Additional owner | Christoph Peters, Institut fuer Molekulare Medizin und Zellforschung, Albert-Ludwigs-Universitaet Freiburg, Freiburg, Germany |
| Genetic information | A neomycin selection cassette was inserted into a BglII site in exon 4. Northern blot analysis of total RNA showed a lack of transcript in homozygous mutant mice. An absence of protein in homozygous mutant mice was determined by Western blot analysis of kidney lysosomal protein extracts. The proteolytic activity of the endogenous protein was found to be absent using an assay involving liver lysosomal extracts obtained from of homozygous mutant mice and a fluorogenic substrate. |
| Phenotypic information | Loss of cathepsin B protects from TNF-alpha induced apoptosis in hepatocytes and from acute pancreatitis in an experimental pancreatitis model. |
| Breeding history | Mice were backcrossed to C57BL/6J for at least 15 generations. Currently the strain is inbred. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6J |
