B6.Cg-Tg(Gfap-tTA)7Igta Tg(tetO-Ppp3ca*)5Igta/Cnbc
| Status | Available to order |
| EMMA ID | EM:06201 |
| Citation information | RRID:IMSR_EM:06201 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Gfap-tTA)7Igta Tg(tetO-Ppp3ca*)5Igta/Cnbc |
| Alternative name | AIC (astrocyte inducible calcineurin) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gfap-tTA)7Igta, Tg(tetO-Ppp3ca*)5Igta |
| Gene/Transgene symbol | Tg(Gfap-tTA)7Igta, Tg(tetO-Ppp3ca*)5Igta |
Information from provider
| Provider | Ana M. Fernandez |
| Provider affiliation | Functional and Systems Neurobiology, Instituto Cajal, CSIC |
| Genetic information | Regulated expression of a truncated, constitutively active form of calcineurin (CnA or Ppp3ca) in astrocytes was achieved with the Tet-off system by generating two transgenic mouse lines. A first mouse line expressed tet-transactivator (tTA) under the astrocyte-specific GFAP promoter. A second transgenic line expressed CnA under the control of the TetO promoter. A cDNA encoding a truncated form of the murine calcineurin catalytic subunit A, CnA (OKeefe et al., 1992), was placed downstream from the TetO promoter (from pTRE vector; Clontech). The pGFAPtTA and TetOCnA DNA fragments were purified and independently injected into fertilized eggs of C57BL/6 x SJL mice. Founder mice were analyzed by Southern blotting and PCR (see Fernandez et al., 2001), using specific primers and backcrossed to C57BL/6 mice to generate the lines pGFAPtTA (GFAP) and TetOCnA (CnA). The astrocyte inducible calcineurin (AIC) mice were generated by crossing the GFAP7 (F3) with the CnA5 (F3) mice. |
| Phenotypic information | Calcineurin inducible inflammatory phenotype in astrocytes. |
| Breeding history | The AIC strain was backcrossed to C57BL/6 for 10 generations. It is currently bred as homozygous male x homozygous female. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6 males, homozygous C57BL/6 females |
| Stage of embryos | 2-cell |
Literature references
- Calcineurin in reactive astrocytes plays a key role in the interplay between proinflammatory and anti-inflammatory signals.;Fernandez Ana M, Fernandez Silvia, Carrero Paloma, Garcia-Garcia Miguel, Torres-Aleman Ignacio, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;8745-56; 17699657