B6.Cg-Tg(Gfap-tTA)7Igta Tg(tetO-Ppp3ca*)5Igta/Cnbc
| Status | Available to order |
| EMMA ID | EM:06201 |
| International strain name | B6.Cg-Tg(Gfap-tTA)7Igta Tg(tetO-Ppp3ca*)5Igta/Cnbc |
| Alternative name | AIC (astrocyte inducible calcineurin) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gfap-tTA)7Igta, Tg(tetO-Ppp3ca*)5Igta |
| Gene/Transgene symbol | Tg(Gfap-tTA)7Igta, Tg(tetO-Ppp3ca*)5Igta |
Information from provider
| Provider | Ana M. Fernandez |
| Provider affiliation | Functional and Systems Neurobiology, Instituto Cajal, CSIC |
| Genetic information | Regulated expression of a truncated, constitutively active form of calcineurin (CnA or Ppp3ca) in astrocytes was achieved with the Tet-off system by generating two transgenic mouse lines. A first mouse line expressed tet-transactivator (tTA) under the astrocyte-specific GFAP promoter. A second transgenic line expressed CnA under the control of the TetO promoter. A cDNA encoding a truncated form of the murine calcineurin catalytic subunit A, CnA (OKeefe et al., 1992), was placed downstream from the TetO promoter (from pTRE vector; Clontech). The pGFAPtTA and TetOCnA DNA fragments were purified and independently injected into fertilized eggs of C57BL/6 x SJL mice. Founder mice were analyzed by Southern blotting and PCR (see Fernandez et al., 2001), using specific primers and backcrossed to C57BL/6 mice to generate the lines pGFAPtTA (GFAP) and TetOCnA (CnA). The astrocyte inducible calcineurin (AIC) mice were generated by crossing the GFAP7 (F3) with the CnA5 (F3) mice. |
| Phenotypic information | Calcineurin inducible inflammatory phenotype in astrocytes. |
| Breeding history | The AIC strain was backcrossed to C57BL/6 for 10 generations. It is currently bred as homozygous male x homozygous female. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6, homozygous C57BL/6 |
| Stage of embryos | 2-cell |
Literature references
- Calcineurin in reactive astrocytes plays a key role in the interplay between proinflammatory and anti-inflammatory signals.;Fernandez Ana M, Fernandez Silvia, Carrero Paloma, Garcia-Garcia Miguel, Torres-Aleman Ignacio, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;8745-56; 17699657