B6Brd;B6N-Tyr^c-Brd A^tm1Brd Usp42^{tm2a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:06128
International strain name	B6Brd;B6N-Tyr^c-Brd A^tm1Brd Usp42^{tm2a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0130_1_D07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Usp42^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Usp42
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0130_1_D07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac
Breeding at archiving centre	Frozen samples received. No breeding at archiving centre. Due to the possible presence of C57BL/6N-A^tm1Brd/a, and C57BL/6J-Tyr^c-Brd, may produce non-agouti, agouti or albino pups.

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased body length / IMPC
decreased body weight / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
hyperactivity / IMPC
male infertility / IMPC
decreased lean body mass / IMPC
abnormal behavior / IMPC

IMPC phenotypes (gene matching)

decreased body weight / IMPC
abnormal behavior / IMPC
hyperactivity / IMPC
decreased body length / IMPC
decreased lean body mass / IMPC
cataract / IMPC
abnormal lens morphology / IMPC
male infertility / IMPC

MGI phenotypes (allele matching)

abnormal lens morphology / MGI
cataract / MGI
hyperactivity / MGI
abnormal fertility/fecundity / MGI
oligozoospermia / MGI
abnormal behavior / MGI
increased T cell number / MGI
increased CD4-positive, alpha beta T cell number / MGI
testis degeneration / MGI
decreased body length / MGI
decreased body weight / MGI
thrombocytopenia / MGI
decreased lean body mass / MGI
decreased lumbar vertebrae number / MGI
increased sacral vertebrae number / MGI

MGI phenotypes (gene matching)

decreased body length / MGI
decreased body weight / MGI
abnormal lens morphology / MGI
cataract / MGI
hyperactivity / MGI
abnormal fertility/fecundity / MGI
oligozoospermia / MGI
thrombocytopenia / MGI
decreased lean body mass / MGI
decreased lumbar vertebrae number / MGI
abnormal behavior / MGI
increased T cell number / MGI
increased CD4-positive, alpha beta T cell number / MGI
increased sacral vertebrae number / MGI
testis degeneration / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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