C57BL/6N-A^tm1Brd Cbx6^{tm1a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:06102
International strain name	C57BL/6N-A^tm1Brd Cbx6^{tm1a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0445_7_A03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cbx6^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cbx6
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0445_7_A03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased circulating HDL cholesterol level / IMPC
abnormal tibia morphology / IMPC
increased circulating chloride level / IMPC
increased lean body mass / IMPC
decreased circulating cholesterol level / IMPC
increased circulating creatinine level / IMPC
decreased circulating glucose level / IMPC
increased blood urea nitrogen level / IMPC
increased circulating sodium level / IMPC
increased circulating amylase level / IMPC
decreased mean platelet volume / IMPC
decreased total body fat amount / IMPC
increased bone mineral content / IMPC
decreased fasting circulating glucose level / IMPC

IMPC phenotypes (gene matching)

increased circulating chloride level / IMPC
increased bone mineral content / IMPC
abnormal tibia morphology / IMPC
increased circulating sodium level / IMPC
decreased fasting circulating glucose level / IMPC
increased circulating amylase level / IMPC
increased lean body mass / IMPC
decreased circulating HDL cholesterol level / IMPC
increased circulating creatinine level / IMPC
increased blood urea nitrogen level / IMPC
decreased mean platelet volume / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating glucose level / IMPC
decreased total body fat amount / IMPC

MGI phenotypes (allele matching)

decreased body weight / MGI
abnormal bile duct morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal liver vasculature morphology / MGI
perimembraneous ventricular septal defect / MGI
absent hypoglossal nerve / MGI
abnormal Mullerian duct topology / MGI
abnormal infrahyoid muscle connection / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery topology / MGI
heterochrony / MGI

MGI phenotypes (gene matching)

decreased body weight / MGI
no abnormal phenotype detected / MGI
abnormal bile duct morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal liver vasculature morphology / MGI
perimembraneous ventricular septal defect / MGI
absent hypoglossal nerve / MGI
abnormal Mullerian duct topology / MGI
abnormal infrahyoid muscle connection / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery topology / MGI
heterochrony / MGI
hemangioma / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6N-Atm1Brd Cbx6tm1a(EUCOMM)Wtsi/WtsiCnbc