CD1(129S)-Tg(ANPEP)861Mmul/Cnbc

Status	Available to order
EMMA ID	EM:06063
International strain name	CD1(129S)-Tg(ANPEP)861Mmul/Cnbc
Alternative name	CD1.129-Stat1<+/+>-Tg(APN)861<+/+>/Cnbc
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(ANPEP)861Mmul, Stat1⁺
Gene/Transgene symbol	Tg(ANPEP)861Mmul, Stat1

Information from provider

Provider	Luis Enjuanes
Provider affiliation	Centro Nacional de Biotecnologia (CNB-CSIC)
Additional owner	There are 14 strains of Stat1 KO available through IMSR, including the one used here from David Levy's lab at NYU (Stat1, MGI:1930947)
Genetic information	Transgenic mice were produced in ICR (CD1) mouse outbred background by standard pronuclear microinjection and bred to homozygosity. Stat1 KO mice were produced and donated by David Levy (NYU) in 129S/Sv (CCE ES cells) background and backcrossed to CD1 mouse background to homozygosity. A neomycin resistance cassette replaced 5.7 kb of sequence, including 3 exons and a portion of a fourth encoding amino acids 221-365. This allele produces a partial nonfunctional protein product. Both mouse lines were bred to double homozygosity in CD1 background. Line 270 and 861 expressed the highest levels of APN. These mice are double homozygous for Tg APN line 861 and double homozygous for Stat1 wild-type allele.
Phenotypic information	Transgenic APN mice that are susceptible to human coronavirus 229E infection
Breeding history	Stat1 KO mice originally generated in 129S/Sv background (CCE ES cells) and backcrossed to CD1. Tg APN mice generated in CD1(ICR) mice. Both mouse lines bred to double homozygous line. In this case Stat1 locus carries both wild-type alleles
References	Development of a transgenic mouse model susceptible to human coronavirus 229E.;Lassnig Caroline, Sanchez Carlos M, Egerbacher Monika, Walter Ingrid, Majer Susanne, Kolbe Thomas, Pallares Pilar, Enjuanes Luis, Müller Mathias, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;8275-80; 15919828 Targeted disruption of the mouse Stat1 gene results in compromised innate immunity to viral disease.;Durbin J E, Hackenmiller R, Simon M C, Levy D E, ;1996;Cell;84;443-50; 8608598
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autoimmune enteropathy and endocrinopathy-susceptibility to chronic infections syndrome / Orphanet_391487
Susceptibility to viral and mycobacterial infections due to STAT1 deficiency / Orphanet_391311
Mendelian susceptibility to mycobacterial diseases due to partial STAT1 deficiency / Orphanet_319595

IMPC phenotypes (gene matching)

increased monocyte cell number / IMPC
decreased mean corpuscular hemoglobin / IMPC
increased neutrophil cell number / IMPC
increased lymphocyte cell number / IMPC
abnormal gait / IMPC
impaired righting response / IMPC
decreased mean corpuscular volume / IMPC
enlarged lymph nodes / IMPC
decreased lymphocyte cell number / IMPC
decreased bone mineral content / IMPC
increased leukocyte cell number / IMPC
decreased circulating alkaline phosphatase level / IMPC
increased large unstained cell number / IMPC
increased eosinophil cell number / IMPC
increased basophil cell number / IMPC

MGI phenotypes (gene matching)

abnormal intestine morphology / MGI
abnormal tibia morphology / MGI
decreased body size / MGI
abnormal cornea morphology / MGI
multifocal hepatic necrosis / MGI
abnormal T cell activation / MGI
decreased level of surface class I molecules / MGI
increased mammary adenocarcinoma incidence / MGI
no abnormal phenotype detected / MGI
increased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal leukocyte physiology / MGI
abnormal macrophage physiology / MGI
increased IgG level / MGI
increased myocardial infarction size / MGI
abnormal cell cycle / MGI
abnormal redox activity / MGI
increased cardiomyocyte apoptosis / MGI
abnormal nitric oxide homeostasis / MGI
increased compact bone thickness / MGI
decreased B-1 B cell number / MGI
increased osteoclast cell number / MGI
increased bone resorption / MGI
decreased B cell number / MGI
abnormal response to infection / MGI
abnormal MHC II cell surface expression on macrophages / MGI
impaired natural killer cell mediated cytotoxicity / MGI
immune system phenotype / MGI
vision/eye phenotype / MGI
abnormal T-helper 2 physiology / MGI
increased bone mass / MGI
abnormal cell physiology / MGI
abnormal common myeloid progenitor cell morphology / MGI
increased NK T cell number / MGI
decreased NK cell number / MGI
increased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
decreased single-positive T cell number / MGI
decreased susceptibility to neuronal excitotoxicity / MGI
abnormal osteoblast differentiation / MGI
abnormal osteoclast differentiation / MGI
decreased interferon-gamma secretion / MGI
increased circulating interferon-gamma level / MGI
decreased circulating interferon-gamma level / MGI
increased circulating interleukin-12b level / MGI
decreased interleukin-1 beta secretion / MGI
decreased susceptibility to endotoxin shock / MGI
decreased NK cell degranulation / MGI
decreased hepatocyte apoptosis / MGI
decreased sensitivity to induced cell death / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
tumor regression / MGI
abnormal NK cell physiology / MGI
increased effector memory CD8-positive, alpha-beta T cell number / MGI
increased trabecular bone volume / MGI
increased compact bone volume / MGI
increased macrophage nitric oxide production / MGI
lethality at weaning, complete penetrance / MGI
abnormal CD8-positive, naive alpha-beta T cell number / MGI

Literature references

Development of a transgenic mouse model susceptible to human coronavirus 229E.;Lassnig Caroline, Sanchez Carlos M, Egerbacher Monika, Walter Ingrid, Majer Susanne, Kolbe Thomas, Pallares Pilar, Enjuanes Luis, Müller Mathias, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;8275-80; 15919828
Targeted disruption of the mouse Stat1 gene results in compromised innate immunity to viral disease.;Durbin J E, Hackenmiller R, Simon M C, Levy D E, ;1996;Cell;84;443-50; 8608598

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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