- increased monocyte cell number / IMPC
- decreased mean corpuscular hemoglobin / IMPC
- increased neutrophil cell number / IMPC
- increased lymphocyte cell number / IMPC
- abnormal gait / IMPC
- impaired righting response / IMPC
- decreased mean corpuscular volume / IMPC
- enlarged lymph nodes / IMPC
- decreased lymphocyte cell number / IMPC
- decreased bone mineral content / IMPC
- increased leukocyte cell number / IMPC
- decreased circulating alkaline phosphatase level / IMPC
- increased large unstained cell number / IMPC
- increased eosinophil cell number / IMPC
- increased basophil cell number / IMPC
CD1.Cg-Stat1tm1Dlv Tg(ANPEP)270Mmul/Cnbc
Status | Available to order |
EMMA ID | EM:06060 |
International strain name | CD1.Cg-Stat1tm1Dlv Tg(ANPEP)270Mmul/Cnbc |
Alternative name | CD1.129-Stat1 |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(ANPEP)270Mmul, Stat1tm1Dlv |
Gene/Transgene symbol | Tg(ANPEP)270Mmul, Stat1 |
Information from provider
Provider | Luis Enjuanes |
Provider affiliation | Centro Nacional de Biotecnologia (CNB-CSIC) |
Additional owner | There are 14 strains of Stat1 KO available through IMSR, including the one used here from David Levy's lab at NYU (Stat1 |
Genetic information | Transgenic mice were produced in CD1 (ICR) outbred background by standard pronuclear microinjection and bred to homozygosity. Stat1 KO mice were produced and donated by David Levy (NYU) in 129S/Sv (CCE ES cells) background and backcrossed to CD1 mouse background to homozygosity. A neomycin resistance cassette replaced 5.7 kb of sequence, including 3 exons and a portion of a fourth encoding amino acids 221-365. This allele produces a partial nonfunctional protein product. Both mouse lines were bred to double homozygosity in CD1 background. Line 270 and 861 expressed the highest levels of aminopeptidase N (APN). These mice are double homozygous for Tg APN line 270 and double homozygous for Stat1 KO mutation. |
Phenotypic information | Immunocompromised (due to Stat1 deficit) mice that are susceptible to human coronavirus 229E |
Breeding history | Stat1 KO mice originally generated in 129S/Sv background (CCE ES cells) and backcrossed to CD1. Tg APN mice generated in CD1 (ICR) mice. Bred to a double homozygous line. |
References |
|
Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | yes |
Immunocompromised | yes |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autoimmune enteropathy and endocrinopathy-susceptibility to chronic infections syndrome / Orphanet_391487
- Susceptibility to viral and mycobacterial infections due to STAT1 deficiency / Orphanet_391311
- Mendelian susceptibility to mycobacterial diseases due to partial STAT1 deficiency / Orphanet_319595
IMPC phenotypes (gene matching)
MGI phenotypes (allele matching)
- increased mammary adenocarcinoma incidence / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- increased circulating interferon-gamma level / MGI
- increased circulating interleukin-12b level / MGI
- abnormal NK cell physiology / MGI
- decreased level of surface class I molecules / MGI
- increased susceptibility to bacterial infection / MGI
- increased susceptibility to viral infection / MGI
- abnormal MHC II cell surface expression on macrophages / MGI
- vision/eye phenotype / MGI
- decreased susceptibility to endotoxin shock / MGI
- abnormal intestine morphology / MGI
- decreased body size / MGI
- multifocal hepatic necrosis / MGI
- abnormal leukocyte physiology / MGI
- lethality at weaning, complete penetrance / MGI
MGI phenotypes (gene matching)
- abnormal intestine morphology / MGI
- abnormal tibia morphology / MGI
- decreased body size / MGI
- abnormal cornea morphology / MGI
- multifocal hepatic necrosis / MGI
- abnormal T cell activation / MGI
- decreased level of surface class I molecules / MGI
- increased mammary adenocarcinoma incidence / MGI
- no abnormal phenotype detected / MGI
- increased susceptibility to bacterial infection / MGI
- increased susceptibility to viral infection / MGI
- abnormal leukocyte physiology / MGI
- abnormal macrophage physiology / MGI
- increased IgG level / MGI
- increased myocardial infarction size / MGI
- abnormal cell cycle / MGI
- abnormal redox activity / MGI
- increased cardiomyocyte apoptosis / MGI
- abnormal nitric oxide homeostasis / MGI
- increased compact bone thickness / MGI
- decreased B-1 B cell number / MGI
- increased osteoclast cell number / MGI
- increased bone resorption / MGI
- decreased B cell number / MGI
- abnormal response to infection / MGI
- abnormal MHC II cell surface expression on macrophages / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- immune system phenotype / MGI
- vision/eye phenotype / MGI
- abnormal T-helper 2 physiology / MGI
- increased bone mass / MGI
- abnormal cell physiology / MGI
- abnormal common myeloid progenitor cell morphology / MGI
- increased NK T cell number / MGI
- decreased NK cell number / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- decreased single-positive T cell number / MGI
- decreased susceptibility to neuronal excitotoxicity / MGI
- abnormal osteoblast differentiation / MGI
- abnormal osteoclast differentiation / MGI
- decreased interferon-gamma secretion / MGI
- increased circulating interferon-gamma level / MGI
- decreased circulating interferon-gamma level / MGI
- increased circulating interleukin-12b level / MGI
- decreased interleukin-1 beta secretion / MGI
- decreased susceptibility to endotoxin shock / MGI
- decreased NK cell degranulation / MGI
- decreased hepatocyte apoptosis / MGI
- decreased sensitivity to induced cell death / MGI
- increased susceptibility to bacterial infection induced morbidity/mortality / MGI
- tumor regression / MGI
- abnormal NK cell physiology / MGI
- increased effector memory CD8-positive, alpha-beta T cell number / MGI
- increased trabecular bone volume / MGI
- increased compact bone volume / MGI
- increased macrophage nitric oxide production / MGI
- lethality at weaning, complete penetrance / MGI
- abnormal CD8-positive, naive alpha-beta T cell number / MGI
Literature references
- Development of a transgenic mouse model susceptible to human coronavirus 229E.;Lassnig Caroline, Sanchez Carlos M, Egerbacher Monika, Walter Ingrid, Majer Susanne, Kolbe Thomas, Pallares Pilar, Enjuanes Luis, Müller Mathias, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;8275-80; 15919828
- Targeted disruption of the mouse Stat1 gene results in compromised innate immunity to viral disease.;Durbin J E, Hackenmiller R, Simon M C, Levy D E, ;1996;Cell;84;443-50; 8608598
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