C57BL/6NTac-Stk39^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:06040
International strain name	C57BL/6NTac-Stk39^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0169_4_D02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Stk39^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Stk39
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0169_4_D02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6NTac
Breeding at archiving centre	Frozen samples received. No breeding at archiving centre.

Disease and phenotype information

IMPC phenotypes (allele matching)

increased bone mineral density / IMPC
increased circulating calcium level / IMPC
decreased body weight / IMPC
decreased mean corpuscular volume / IMPC
decreased circulating chloride level / IMPC
thrombocytosis / IMPC

IMPC phenotypes (gene matching)

increased circulating calcium level / IMPC
increased bone mineral density / IMPC
decreased body weight / IMPC
thrombocytosis / IMPC
decreased mean corpuscular volume / IMPC
decreased circulating chloride level / IMPC

MGI phenotypes (gene matching)

hypotension / MGI
reduced fertility / MGI
abnormal artery morphology / MGI
increased circulating aldosterone level / MGI
decreased urine calcium level / MGI
no phenotypic analysis / MGI
decreased vasoconstriction / MGI
decreased heart left ventricle weight / MGI
hypokalemia / MGI
abnormal neuron physiology / MGI
decreased mean systemic arterial blood pressure / MGI
decreased systemic vascular resistance / MGI
decreased heart rate / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
increased urine potassium level / MGI
decreased circulating potassium level / MGI
decreased systemic arterial diastolic blood pressure / MGI
decreased systemic arterial systolic blood pressure / MGI
increased urine sodium level / MGI
decreased urine sodium level / MGI
abnormal blood homeostasis / MGI
decreased circulating magnesium level / MGI
increased urine magnesium level / MGI
decreased urine chloride ion level / MGI
abnormal heart echocardiography feature / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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