129S5;B6N-Ywhaetm1e(EUCOMM)Wtsi/WtsiBiat

Status

Available to order

EMMA IDEM:06036
International strain name129S5;B6N-Ywhaetm1e(EUCOMM)Wtsi/WtsiBiat
Alternative nameEPD0200_5_G01
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolYwhaetm1e(EUCOMM)Wtsi
Gene/Transgene symbolYwhae
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0200_5_G01. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal heart morphology / MGI
  • thin ventricular wall / MGI
  • abnormal cerebral cortex morphology / MGI
  • cyanosis / MGI
  • decreased litter size / MGI
  • abnormal brain morphology / MGI
  • abnormal myocardial trabeculae morphology / MGI
  • abnormal cell cycle / MGI
  • abnormal heart left ventricle morphology / MGI
  • thin myocardium compact layer / MGI
  • decreased mitotic index / MGI
  • abnormal heart ventricle morphology / MGI
  • abnormal neuronal migration / MGI
  • dilated heart ventricle / MGI
  • abnormal hippocampus pyramidal cell layer / MGI
  • ventricular septal defect / MGI
  • thin ventricle myocardium compact layer / MGI
  • perinatal lethality, complete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • abnormal fetal cardiomyocyte physiology / MGI
  • decreased fetal cardiomyocyte proliferation / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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