C57BL/6NTac-Bbs5^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:06028
International strain name	C57BL/6NTac-Bbs5^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0227_3_C06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Bbs5^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Bbs5
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0227_3_C06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Bardet-Biedl syndrome / Orphanet_110

IMPC phenotypes (gene matching)

increased circulating glucose level / IMPC
decreased bone mineral content / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased lean body mass / IMPC
impaired glucose tolerance / IMPC
abnormal bone structure / IMPC
abnormal gait / IMPC
increased circulating fructosamine level / IMPC
increased total body fat amount / IMPC
decreased grip strength / IMPC
decreased bone mineral density / IMPC
increased circulating aspartate transaminase level / IMPC
thrombocytopenia / IMPC
short tibia / IMPC
decreased respiratory quotient / IMPC
decreased circulating triglyceride level / IMPC
increased monocyte cell number / IMPC
increased leukocyte cell number / IMPC
decreased heart weight / IMPC
increased hematocrit / IMPC
increased circulating alanine transaminase level / IMPC
increased fasting circulating glucose level / IMPC
increased hemoglobin content / IMPC
abnormal retina morphology / IMPC
increased lymphocyte cell number / IMPC
increased mean corpuscular volume / IMPC
tremors / IMPC

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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