C57BL/6NTac-Ptpn22tm1e(EUCOMM)Wtsi/Cnrm

Status

Available to order

EMMA IDEM:06015
International strain nameC57BL/6NTac-Ptpn22tm1e(EUCOMM)Wtsi/Cnrm
Alternative nameEPD0203_5_A05
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolPtpn22tm1e(EUCOMM)Wtsi
Gene/Transgene symbolPtpn22
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider CNR, Consiglio Nazionale delle Ricerche
Provider affiliationEMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0203_5_A05. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal salivary gland morphology / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • enlarged lymph nodes / MGI
  • abnormal thymus morphology / MGI
  • thymus hyperplasia / MGI
  • enlarged thymus / MGI
  • abnormal lung morphology / MGI
  • abnormal T cell activation / MGI
  • autoimmune response / MGI
  • increased inflammatory response / MGI
  • vasculitis / MGI
  • premature death / MGI
  • abnormal B cell differentiation / MGI
  • abnormal T cell differentiation / MGI
  • abnormal dendritic cell physiology / MGI
  • abnormal Peyer's patch germinal center morphology / MGI
  • abnormal double-positive T cell morphology / MGI
  • abnormal T cell physiology / MGI
  • abnormal B cell number / MGI
  • abnormal B cell physiology / MGI
  • increased IgM level / MGI
  • increased IgE level / MGI
  • glomerulonephritis / MGI
  • increased anti-insulin autoantibody level / MGI
  • abnormal thymocyte activation / MGI
  • abnormal T cell subpopulation ratio / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • abnormal B cell morphology / MGI
  • increased regulatory T cell number / MGI
  • increased B cell number / MGI
  • increased T cell number / MGI
  • increased double-positive T cell number / MGI
  • increased B cell proliferation / MGI
  • increased T cell proliferation / MGI
  • immune system phenotype / MGI
  • abnormal T cell number / MGI
  • abnormal T cell morphology / MGI
  • increased memory T cell number / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • increased dendritic cell number / MGI
  • increased follicular B cell number / MGI
  • decreased follicular B cell number / MGI
  • increased germinal center B cell number / MGI
  • decreased mature B cell number / MGI
  • abnormal B cell activation / MGI
  • increased spleen germinal center number / MGI
  • increased spleen germinal center size / MGI
  • increased IgG1 level / MGI
  • increased IgG2a level / MGI
  • increased IgG3 level / MGI
  • increased interleukin-12 secretion / MGI
  • increased interleukin-2 secretion / MGI
  • decreased B cell apoptosis / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • increased transitional stage T1 B cell number / MGI
  • increased CD4-positive, alpha-beta memory T cell number / MGI
  • increased effector memory CD4-positive, alpha-beta T cell number / MGI
  • increased effector memory CD8-positive, alpha-beta T cell number / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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