B6;CBA-Tg(CAMK2A-KCNIP3*,-lacZ)33Cnbc/Cnbc

Status

Available to order

EMMA IDEM:06006
International strain nameB6;CBA-Tg(CAMK2A-KCNIP3*,-lacZ)33Cnbc/Cnbc
Alternative nameB6CBA-Tg(hCaMKIIalpha-hEFmDREAM-IRES-lacZ)JN33Cnbc
Strain typeTransgenic Strains
Allele/Transgene symbolTg(CAMK2A-KCNIP3*,-lacZ)33Cnbc
Gene/Transgene symbolTg(CAMK2A-KCNIP3*,-lacZ)33Cnbc

Information from provider

ProviderJose Ramon Naranjo
Provider affiliationCentro Nacional de Biotecnologia (CNB-CSIC)
Genetic informationThe cDNA encoding human KCNIP3 (DREAM) with two amino-acid substitutions at EF-hands 2, 3 and 4 (EFmDREAM) was inserted downstream of the human CaMKIIalpha promoter in a bicistronic expression vector containing an IRES and the lacZ reporter gene. The transgene cassette was microinjected into the pronuclei of one-cell embryos (B6CBA) and maintained as hemizygotes in mixed background B6CBA.
Phenotypic informationModerate to low transgene expression, brain, immune system and reproductive system, expression not detected in the entire spinal cord. The presence of lacZ reporter gene cassette within these transgenes makes them prone to display alterations in the expected gene expression pattern and levels.
Breeding historyGenerated in B6CBA background and maintained as hemizygous in mixed B6CBA background.
References
  • Downstream regulatory element antagonist modulator regulates Ca2+ homeostasis and viability in cerebellar neurons.;Gomez-Villafuertes Rosa, Torres Begoña, Barrio Jorge, Savignac Magali, Gabellini Nadia, Rizzato Federico, Pintado Belen, Gutierrez-Adan Alfonso, Mellström Britt, Carafoli Ernesto, Naranjo Jose R, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;10822-30; 16306395
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous (C57BL/6 x CBA)F2, homozygous (C57BL/6 x CBA)F2
Stage of embryos2-cell

Literature references

  • Downstream regulatory element antagonist modulator regulates Ca2+ homeostasis and viability in cerebellar neurons.;Gomez-Villafuertes Rosa, Torres Begoña, Barrio Jorge, Savignac Magali, Gabellini Nadia, Rizzato Federico, Pintado Belen, Gutierrez-Adan Alfonso, Mellström Britt, Carafoli Ernesto, Naranjo Jose R, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;10822-30; 16306395

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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