C57BL/6N-A^tm1Brd Mrap2^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:05973
Citation information	RRID:IMSR_EM:05973 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Mrap2^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0424_6_B02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Mrap2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Mrap2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0424_6_B02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
increased circulating insulin level / IMPC
increased circulating alkaline phosphatase level / IMPC
abnormal bone structure / IMPC
decreased lean body mass / IMPC
impaired glucose tolerance / IMPC
increased total body fat amount / IMPC
increased red blood cell distribution width / IMPC
decreased bone mineral content / IMPC
increased fasting circulating glucose level / IMPC

IMPC phenotypes (gene matching)

abnormal bone structure / IMPC
impaired glucose tolerance / IMPC
increased total body fat amount / IMPC
increased circulating calcium level / IMPC
decreased bone mineral content / IMPC
increased fasting circulating glucose level / IMPC
decreased lean body mass / IMPC
increased red blood cell distribution width / IMPC
increased circulating insulin level / IMPC
increased circulating alkaline phosphatase level / IMPC

MGI phenotypes (gene matching)

increased brown adipose tissue amount / MGI
increased body length / MGI
increased body weight / MGI
obese / MGI
polyphagia / MGI
abnormal intestinal absorption / MGI
hepatic steatosis / MGI
decreased lean body mass / MGI
abnormal adrenaline level / MGI
abnormal noradrenaline level / MGI
homeostasis/metabolism phenotype / MGI
behavior/neurological phenotype / MGI
increased susceptibility to weight gain / MGI
increased percent body fat/body weight / MGI
increased circulating leptin level / MGI
increased white fat cell size / MGI
increased total body fat amount / MGI
increased food intake / MGI
increased compensatory feeding amount / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Mrap2tm1a(EUCOMM)Wtsi/WtsiBiat