C57BL/6N-A^tm1Brd Arpc1b^{tm1a(EUCOMM)Wtsi}/WtsiBiat

Status	Available to order
EMMA ID	EM:05965
International strain name	C57BL/6N-A^tm1Brd Arpc1b^{tm1a(EUCOMM)Wtsi}/WtsiBiat
Alternative name	EPD0423_1_B04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Arpc1b^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Arpc1b
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0423_1_B04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
increased leukocyte cell number / IMPC
decreased body length / IMPC
increased mean platelet volume / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased macrophage cell number / IMPC
vertebral fusion / IMPC
fusion of vertebral arches / IMPC
decreased circulating serum albumin level / IMPC
thrombocytosis / IMPC
decreased mean corpuscular hemoglobin / IMPC
increased circulating total protein level / IMPC
decreased NK cell number / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
increased plasma cell number / IMPC
decreased transitional stage T2 B cell number / IMPC
increased red blood cell distribution width / IMPC
decreased circulating fructosamine level / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
decreased KLRG1-positive NK cell number / IMPC
increased KLRG1+ CD4 alpha-beta T cell number / IMPC
increased memory-marker NK cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
increased CD103-positive CD11b-low dendritic cell number / IMPC
increased immature NK cell number / IMPC
decreased mature NK cell number / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
decreased marginal zone precursor B cell number / IMPC
increased effector memory T-helper cell number / IMPC
increased KLRG1-positive T-helper cell number / IMPC
atrophy / IMPC
process of degenerative change / IMPC
glomerulonephritis / IMPC
vasculitis / IMPC
inflammation / IMPC

IMPC phenotypes (gene matching)

increased CD4-positive, alpha-beta memory T cell number / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased mean corpuscular hemoglobin / IMPC
increased effector memory T-helper cell number / IMPC
increased leukocyte cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
process of degenerative change / IMPC
increased KLRG1-positive T-helper cell number / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
increased red blood cell distribution width / IMPC
increased circulating calcium level / IMPC
decreased mature NK cell number / IMPC
decreased marginal zone precursor B cell number / IMPC
glomerulonephritis / IMPC
decreased body length / IMPC
thrombocytosis / IMPC
decreased circulating serum albumin level / IMPC
atrophy / IMPC
decreased transitional stage T2 B cell number / IMPC
increased KLRG1+ CD4 alpha-beta T cell number / IMPC
increased circulating total protein level / IMPC
increased immature NK cell number / IMPC
increased plasma cell number / IMPC
increased memory-marker NK cell number / IMPC
fusion of vertebral arches / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased KLRG1-positive NK cell number / IMPC
inflammation / IMPC
increased CD103-positive CD11b-low dendritic cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
decreased NK cell number / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased macrophage cell number / IMPC
vertebral fusion / IMPC
vasculitis / IMPC
decreased circulating fructosamine level / IMPC
increased mean platelet volume / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

C57BL/6N-Atm1Brd Arpc1btm1a(EUCOMM)Wtsi/WtsiBiat