MGI phenotypes (gene matching)
B6.Cg-Polr2atm1(cre/ERT2)Bbd/Cnbc
| Status | Available to order |
| EMMA ID | EM:05946 |
| International strain name | B6.Cg-Polr2atm1(cre/ERT2)Bbd/Cnbc |
| Alternative name | Polr2a |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Polr2atm1(cre/ERT2)Bbd |
| Gene/Transgene symbol | Polr2a |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | An IRES-CreERT2 cassette was knocked in between the termination codon and the polyadenylation signal of the Polr2a gene to allow for bicistronic expression of the tamoxifen inducible Cre recombinase CreERT2. This mouse strain is used for inducible ubiquitous Cre recombinase expression. |
| Phenotypic information | No phenotype is associated to this mutation. |
| Breeding history | Chimeras were crossed with CD1 females for germ line transmission analysis. Heterozygous animals were crossed with Tg(ACTFLPe)9205Dym mice to remove the frt-flanked neomycin resistance cassette used for homologous recombination selection. Then the line was backcrossed to C57BL/6J for more than 10 generations. Line has been maintained in homozygosis. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J |
Disease and phenotype information
Literature references
- Mice thrive without Cdk4 and Cdk2.;Barrière Cédric, Santamaría David, Cerqueira Antonio, Galán Javier, Martín Alberto, Ortega Sagrario, Malumbres Marcos, Dubus Pierre, Barbacid Mariano, ;2007;Molecular oncology;1;72-83; 19383288
- Protein farnesyltransferase in embryogenesis, adult homeostasis, and tumor development.;Mijimolle Nieves, Velasco Juan, Dubus Pierre, Guerra Carmen, Weinbaum Carolyn A, Casey Patrick J, Campuzano Victoria, Barbacid Mariano, ;2005;Cancer cell;7;313-24; 15837621
- Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context.;Guerra Carmen, Mijimolle Nieves, Dhawahir Alma, Dubus Pierre, Barradas Marta, Serrano Manuel, Campuzano Victoria, Barbacid Mariano, ;2003;Cancer cell;4;111-20; 12957286
- c-Raf, but not B-Raf, is essential for development of K-Ras oncogene-driven non-small cell lung carcinoma.;Blasco Rafael B, Francoz Sarah, Santamaría David, Cañamero Marta, Dubus Pierre, Charron Jean, Baccarini Manuela, Barbacid Mariano, ;2011;Cancer cell;19;652-63; 21514245
- p38alpha MAP kinase is essential in lung stem and progenitor cell proliferation and differentiation.;Ventura Juan José, Tenbaum Stephan, Perdiguero Eusebio, Huth Marion, Guerra Carmen, Barbacid Mariano, Pasparakis Manolis, Nebreda Angel R, ;2007;Nature genetics;39;750-8; 17468755
- Transcription factor E4F1 is essential for epidermal stem cell maintenance and skin homeostasis.;Lacroix Matthieu, Caramel Julie, Goguet-Rubio Perrine, Linares Laetitia K, Estrach Soline, Hatchi Elodie, Rodier Geneviève, Lledo Gwendaline, de Bettignies Carine, Thépot Amélie, Deraison Céline, Chébli Karim, Hovnanian Alain, Hainaut Pierre, Dubus Pierre, Sardet Claude, Le Cam Laurent, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;21076-81; 21088222