129S2.CD1-Tg(Myh6-Htr2b)1Lum/Orl
| Status | Available to order |
| EMMA ID | EM:05938 |
| Citation information | RRID:IMSR_EM:05938 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129S2.CD1-Tg(Myh6-Htr2b)1Lum/Orl |
| Alternative name | MHC-5-HT2B in 129S2 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Myh6-Htr2b)1Lum |
| Gene/Transgene symbol | Tg(Myh6-Htr2b)1Lum |
Information from provider
| Provider | Luc Maroteaux |
| Provider affiliation | Institut du Fer a Moulin, INSERM UMR S-839 |
| Genetic information | A 5.5 Kbp BamHI-KpnI restriction fragment of the mouse alpha-myosin heavy chain (MHC) was fused to a 2.0 Kbp XbaI-XbaI restriction fragment of mouse Htr2b (5-HT2BR) cDNA. The recombinant plasmid p-MHC-5-HT2B was linearized, microinjected into pronuclei of fertilized CD1 mouse eggs and implanted into pseudopregnant CD1 foster mothers. |
| Phenotypic information | The MHC-5-HT2B receptor transgenic mice exhibit hypertrophic heart with increased ventricular mass, abnormal cellular organization and proliferation of mitochondria. This was not accompanied with cardiovascular functional alterations. Seven-fold overexpression of the 5-HT2B receptor in the heart leads to compensated hypertrophic cardiomyopathy associated with proliferation of the mitochondria. This observation suggests a role for mitochondria in the hypertrophic signaling that is regulated by serotonin. |
| Breeding history | The original CD1 transgenic line was backcrossed more than 10 generations over the 129S2 strain and it is currently maintained over this 129S2 background. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129S2/SvPas males, wild-type 129S2/SvPas females |
| Breeding at archiving centre | Some wild-type females provided by the company potentially have a mixed background: B6.CBAx129/SvPas. It is possible that some frozen embryos have the same background. |
| Stage of embryos | 8-cell |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- Overexpression of the serotonin 5-HT2B receptor in heart leads to abnormal mitochondrial function and cardiac hypertrophy.;Nebigil Canan G, Jaffré Fabrice, Messaddeq Nadia, Hickel Pierre, Monassier Laurent, Launay Jean-Marie, Maroteaux Luc, ;2003;Circulation;107;3223-9; 12810613