C57BL/6-Tmc8tm1c(EUCOMM)Wtsi/Ics

Status

Available to order

EMMA IDEM:05924
International strain nameC57BL/6-Tmc8tm1c(EUCOMM)Wtsi/Ics
Alternative nameTmc8 tm1c
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolTmc8tm1c(EUCOMM)Wtsi
Gene/Transgene symbolTmc8
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Institut Clinique de la Souris-ICS
Provider affiliationInstitut Clinique de la Souris-ICS
Genetic informationThis line originates from the C57BL/6N-Tmc8tm1a(EUCOMM)Wtsi/Ics (EMMA ID EM:04942) strain, after breeding with a flp recombinase deleter line, to convert the original targeted allele tm1a (knock-out first allele) into a conditional tm1c allele. For further details on the construction of this clone see the relevant page at the IKMC portal. Removal of the targeting cassette using flp recombinase is required to convert the targeted tm1a into a conditional tm1c allele - more information on conversion to the b,c and d allele forms. Click here for more information on EUCOMM final vectors.
Phenotypic informationCurrently not available. Heterozygous Tmc8 tm1c are viable.
Breeding historyFor production of the Tmc8 tm1c line, Tmc8 tm1a mice were crossed with N6 C57BL/6NTac flp recombinase (i.e. backcrossed 6x with C57BL/6NTac mice; original background C57BL/6J)
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • increased circulating alanine transaminase level / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • increased circulating aspartate transaminase level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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