C57BL/6-Ndufs3tm1c(EUCOMM)Wtsi/Ics

Status

Available to order

EMMA IDEM:05923
International strain nameC57BL/6-Ndufs3tm1c(EUCOMM)Wtsi/Ics
Alternative nameNdufs3 tm1c
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolNdufs3tm1c(EUCOMM)Wtsi
Gene/Transgene symbolNdufs3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis line originates from the C57BL/6NTac-Ndufs3tm1a(EUCOMM)Wtsi/Ics (EMMA ID EM:04157) strain, after breeding with a flp recombinase deleter line, to convert the original targeted tm1a allele (knock-out first allele) into a conditional tm1c allele. For further details on the construction of the tm1a allele see the relevant page at the IMPC portal. Removal of the targeting cassette using flp recombinase is required to convert the targeted tm1a into a conditional tm1c allele - more information on conversion to the b,c and d allele forms. Click here for more information on EUCOMM final vectors.
Phenotypic informationCurrently not available. Heterozygous Ndufs3 tm1c are viable.
Breeding historyFor production of the Ndufs3 tm1c line, Ndufs3 tm1a mice were crossed with N10 C57BL/6NTac flp recombinase (i.e. backcrossed 10x with C57BL/6NTac mice; original background C57BL/6J).
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France
Animals used for archivingheterozygous C57BL/6N Tac, wild-type C57BL/6NTac
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • increased circulating calcium level / IMPC
  • increased heart weight / IMPC
  • increased bone mineral density / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased circulating phosphate level / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • decreased circulating cholesterol level / IMPC
  • hypertrophy / IMPC
  • increased lean body mass / IMPC
  • increased circulating sodium level / IMPC
  • decreased erythrocyte cell number / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased circulating glucose level / IMPC
MGI phenotypes (gene matching)
  • increased heart weight / MGI
  • decreased erythrocyte cell number / MGI
  • increased lean body mass / MGI
  • decreased lactate dehydrogenase level / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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