B6Brd;B6N-Tyr^c-Brd Ide^{tm1a(EUCOMM)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:05902
International strain name	B6Brd;B6N-Tyr^c-Brd Ide^{tm1a(EUCOMM)Wtsi}/WtsiIeg
Alternative name	EPD0158_4_G09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ide^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Ide
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0158_4_G09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal cranium morphology / IMPC
abnormal maxilla morphology / IMPC
abnormal hair texture / IMPC
increased heart weight / IMPC
abnormal incisor morphology / IMPC
increased circulating amylase level / IMPC
decreased total body fat amount / IMPC
process of degenerative change / IMPC
developmental and structural abnormality / IMPC
cyst / IMPC

IMPC phenotypes (gene matching)

developmental and structural abnormality / IMPC
abnormal hair texture / IMPC
abnormal incisor morphology / IMPC
cyst / IMPC
process of degenerative change / IMPC
increased circulating amylase level / IMPC
abnormal cranium morphology / IMPC
decreased total body fat amount / IMPC
increased heart weight / IMPC
abnormal maxilla morphology / IMPC

MGI phenotypes (allele matching)

abnormal cranium morphology / MGI
abnormal maxilla morphology / MGI
decreased body weight / MGI
abnormal hair texture / MGI
increased heart weight / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating cholesterol level / MGI
abnormal incisor morphology / MGI
decreased circulating aspartate transaminase level / MGI
increased circulating amylase level / MGI
decreased total body fat amount / MGI

MGI phenotypes (gene matching)

abnormal cranium morphology / MGI
abnormal maxilla morphology / MGI
decreased body weight / MGI
hyperglycemia / MGI
abnormal hair texture / MGI
increased circulating insulin level / MGI
increased heart weight / MGI
decreased circulating alanine transaminase level / MGI
amyloid beta deposits / MGI
decreased circulating cholesterol level / MGI
impaired glucose tolerance / MGI
abnormal incisor morphology / MGI
decreased circulating aspartate transaminase level / MGI
increased circulating amylase level / MGI
decreased total body fat amount / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality.;Meneses Maria João, Borges Diego O, Dias Tânia R, Martins Fátima O, Oliveira Pedro F, Macedo M Paula, Alves Marco G, ;2019;Molecular and cellular endocrinology;486;11-17; 30807788
Data on metabolic profile of insulin-degrading enzyme knockout mice.;Borges Diego O, Meneses Maria João, Dias Tânia R, Martins Fátima O, Oliveira Pedro F, Alves Marco G, Macedo M Paula, ;2019;Data in brief;25;104023; 31198829

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Idetm1a(EUCOMM)Wtsi/WtsiIeg