B6Brd;B6N-Tyrc-Brd Idetm1a(EUCOMM)Wtsi/WtsiIeg

Status

Available to order

EMMA IDEM:05902
International strain nameB6Brd;B6N-Tyrc-Brd Idetm1a(EUCOMM)Wtsi/WtsiIeg
Alternative nameEPD0158_4_G09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolIdetm1a(EUCOMM)Wtsi
Gene/Transgene symbolIde
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0158_4_G09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal cranium morphology / IMPC
  • abnormal maxilla morphology / IMPC
  • abnormal hair texture / IMPC
  • increased heart weight / IMPC
  • abnormal incisor morphology / IMPC
  • increased circulating amylase level / IMPC
  • decreased total body fat amount / IMPC
  • process of degenerative change / IMPC
  • developmental and structural abnormality / IMPC
  • cyst / IMPC
IMPC phenotypes (gene matching)
  • developmental and structural abnormality / IMPC
  • abnormal hair texture / IMPC
  • abnormal incisor morphology / IMPC
  • cyst / IMPC
  • process of degenerative change / IMPC
  • increased circulating amylase level / IMPC
  • abnormal cranium morphology / IMPC
  • decreased total body fat amount / IMPC
  • increased heart weight / IMPC
  • abnormal maxilla morphology / IMPC
MGI phenotypes (allele matching)
  • abnormal cranium morphology / MGI
  • abnormal maxilla morphology / MGI
  • decreased body weight / MGI
  • abnormal hair texture / MGI
  • increased heart weight / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased circulating cholesterol level / MGI
  • abnormal incisor morphology / MGI
  • decreased circulating aspartate transaminase level / MGI
  • increased circulating amylase level / MGI
  • decreased total body fat amount / MGI
MGI phenotypes (gene matching)
  • abnormal cranium morphology / MGI
  • abnormal maxilla morphology / MGI
  • decreased body weight / MGI
  • hyperglycemia / MGI
  • abnormal hair texture / MGI
  • increased circulating insulin level / MGI
  • increased heart weight / MGI
  • decreased circulating alanine transaminase level / MGI
  • amyloid beta deposits / MGI
  • decreased circulating cholesterol level / MGI
  • impaired glucose tolerance / MGI
  • abnormal incisor morphology / MGI
  • decreased circulating aspartate transaminase level / MGI
  • increased circulating amylase level / MGI
  • decreased total body fat amount / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality.;Meneses Maria João, Borges Diego O, Dias Tânia R, Martins Fátima O, Oliveira Pedro F, Macedo M Paula, Alves Marco G, ;2019;Molecular and cellular endocrinology;486;11-17; 30807788
  • Data on metabolic profile of insulin-degrading enzyme knockout mice.;Borges Diego O, Meneses Maria João, Dias Tânia R, Martins Fátima O, Oliveira Pedro F, Alves Marco G, Macedo M Paula, ;2019;Data in brief;25;104023; 31198829

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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