C57BL/6NTac-Hiratm1a(EUCOMM)Wtsi/WtsiIeg

Status

Available to order

EMMA IDEM:05901
International strain nameC57BL/6NTac-Hiratm1a(EUCOMM)Wtsi/WtsiIeg
Alternative nameEPD0181_1_A05
Strain typeTargeted Mutant Strains
Allele/Transgene symbolHiratm1a(EUCOMM)Wtsi
Gene/Transgene symbolHira
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0181_1_A05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased leukocyte cell number / IMPC
  • abnormal behavior / IMPC
IMPC phenotypes (gene matching)
  • decreased leukocyte cell number / IMPC
  • abnormal behavior / IMPC
MGI phenotypes (allele matching)
  • decreased leukocyte cell number / MGI
  • decreased rib number / MGI
MGI phenotypes (gene matching)
  • decreased leukocyte cell number / MGI
  • abnormal heart morphology / MGI
  • abnormal heart development / MGI
  • abnormal heart tube morphology / MGI
  • abnormal head morphology / MGI
  • abnormal forebrain morphology / MGI
  • abnormal brain development / MGI
  • open neural tube / MGI
  • abnormal blood vessel morphology / MGI
  • irregular heartbeat / MGI
  • abnormal embryo development / MGI
  • abnormal axial mesoderm / MGI
  • incomplete somite formation / MGI
  • abnormal somite shape / MGI
  • failure to gastrulate / MGI
  • decreased embryo size / MGI
  • abnormal embryo turning / MGI
  • abnormal left-right axis patterning / MGI
  • abnormal allantois morphology / MGI
  • embryonic growth arrest / MGI
  • pericardial edema / MGI
  • abnormal developmental patterning / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal neural tube morphology / MGI
  • abnormal primitive streak morphology / MGI
  • abnormal notochord morphology / MGI
  • abnormal pharyngeal arch morphology / MGI
  • no phenotypic analysis / MGI
  • abnormal vitelline vasculature morphology / MGI
  • decreased rib number / MGI
  • kinked neural tube / MGI
  • embryonic growth retardation / MGI
  • caudal body truncation / MGI
  • abnormal midbrain-hindbrain boundary development / MGI
  • failure of heart looping / MGI
  • abnormal embryonic neuroepithelium morphology / MGI
  • abnormal prechordal plate morphology / MGI
  • enlarged allantois / MGI
  • abnormal rostral-caudal axis patterning / MGI
  • embryo phenotype / MGI
  • abnormal neural plate morphology / MGI
  • abnormal hindbrain development / MGI
  • abnormal paraxial mesoderm morphology / MGI
  • abnormal mesendoderm development / MGI
  • failure of chorioallantoic fusion / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • abnormal head mesenchyme morphology / MGI
  • decreased somite size / MGI
  • embryonic-extraembryonic boundary constriction / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
  • The histone H3.3 chaperone HIRA restrains erythroid-biased differentiation of adult hematopoietic stem cells.;Murdaugh Rebecca L, Hoegenauer Kevin A, Kitano Ayumi, Holt Matthew V, Hill Matthew C, Shi Xiangguo, Tiessen Jonathan F, Chapple Richard, Hu Tianyuan, Tseng Yu-Jung, Lin Angelique, Martin James F, Young Nicolas L, Nakada Daisuke, ;2021;Stem cell reports;16;2014-2028; 34242617
  • Haploinsufficiency of the HIRA gene located in the 22q11 deletion syndrome region is associated with abnormal neurodevelopment and impaired dendritic outgrowth.;Jeanne Médéric, Vuillaume Marie-Laure, Ung Dévina C, Vancollie Valerie E, Wagner Christel, Collins Stephan C, Vonwill Sandrine, Haye Damien, Chelloug Nora, Pfundt Rolph, Kummeling Joost, Moizard Marie-Pierre, Marouillat Sylviane, Kleefstra Tjitske, Yalcin Binnaz, Laumonnier Frédéric, Toutain Annick, ;2021;Human genetics;140;885-896; 33417013

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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