B6Brd;B6N-Tyr^c-Brd Gsk3a^{tm1a(EUCOMM)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:05896
International strain name	B6Brd;B6N-Tyr^c-Brd Gsk3a^{tm1a(EUCOMM)Wtsi}/WtsiIeg
Alternative name	EPD0051_1_H11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Gsk3a^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Gsk3a
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0051_1_H11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal snout morphology / IMPC
decreased body length / IMPC
decreased body weight / IMPC
decreased circulating glucose level / IMPC
decreased total body fat amount / IMPC
decreased circulating fructosamine level / IMPC

IMPC phenotypes (gene matching)

abnormal snout morphology / IMPC
decreased circulating fructosamine level / IMPC
improved glucose tolerance / IMPC
decreased mean platelet volume / IMPC
increased circulating alkaline phosphatase level / IMPC
prolonged QRS complex duration / IMPC
abnormal gait / IMPC
decreased startle reflex / IMPC
decreased body weight / IMPC
decreased total body fat amount / IMPC
increased lean body mass / IMPC
decreased body length / IMPC
decreased exploration in new environment / IMPC
decreased circulating glucose level / IMPC
decreased lean body mass / IMPC

MGI phenotypes (allele matching)

craniofacial phenotype / MGI
wide metopic suture / MGI
decreased body length / MGI
decreased body weight / MGI
decreased circulating glycerol level / MGI
decreased lumbar vertebrae number / MGI
decreased circulating glucose level / MGI
decreased total body fat amount / MGI
increased sacral vertebrae number / MGI

MGI phenotypes (gene matching)

decreased body length / MGI
decreased body weight / MGI
no abnormal phenotype detected / MGI
increased insulin sensitivity / MGI
no phenotypic analysis / MGI
decreased circulating glycerol level / MGI
increased lean body mass / MGI
decreased lumbar vertebrae number / MGI
improved glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI
craniofacial phenotype / MGI
decreased percent body fat/body weight / MGI
decreased circulating glucose level / MGI
decreased total body fat amount / MGI
increased sacral vertebrae number / MGI
increased liver glycogen level / MGI
wide metopic suture / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Gsk3atm1a(EUCOMM)Wtsi/WtsiIeg