C57BL/6N-A^tm1Brd Cfh^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:05871
International strain name	C57BL/6N-A^tm1Brd Cfh^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0424_5_A05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cfh^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cfh
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0424_5_A05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6NTac
Breeding at archiving centre	Frozen material received. Due to the possible presence of C57BL/6N-A^tm1Brd/a, may produce agouti pups.

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating free fatty acids level / IMPC
increased mean corpuscular hemoglobin / IMPC
inflammation / IMPC

IMPC phenotypes (gene matching)

inflammation / IMPC
increased mean corpuscular hemoglobin / IMPC
increased circulating free fatty acids level / IMPC

MGI phenotypes (allele matching)

increased mean corpuscular hemoglobin / MGI

MGI phenotypes (gene matching)

abnormal retinal photoreceptor morphology / MGI
abnormal retina morphology / MGI
premature death / MGI
glomerulonephritis / MGI
albuminuria / MGI
increased urine protein level / MGI
amyloid beta deposits / MGI
abnormal retinal layer morphology / MGI
hematuria / MGI
abnormal retinal pigment epithelium morphology / MGI
abnormal Bruch membrane morphology / MGI
abnormal renal glomerulus morphology / MGI
abnormal circulating protein level / MGI
increased mean corpuscular hemoglobin / MGI
increased susceptibility to type III hypersensitivity reaction / MGI
abnormal retinal rod bipolar cell morphology / MGI
decreased visual acuity / MGI
disorganized photoreceptor outer segment / MGI
abnormal kidney capillary morphology / MGI
abnormal renal glomerulus basement membrane morphology / MGI
expanded mesangial matrix / MGI
mesangial cell hyperplasia / MGI
mesangial cell interposition / MGI
abnormal complement protein level / MGI
decreased a wave amplitude / MGI
decreased b wave amplitude / MGI
decreased circulating complement protein level / MGI
renal glomerular protein deposits / MGI
renal glomerular immunoglobulin deposits / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

Search

INFRAFRONTIER^® and European Mouse Mutant Archive - EMMA^® are registered trademarks at the European Union Intellectual Property Office (EUIPO).