C57BL/6NTac-Kcne2tm1a(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:05862
International strain nameC57BL/6NTac-Kcne2tm1a(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0156_2_F10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolKcne2tm1a(EUCOMM)Wtsi
Gene/Transgene symbolKcne2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0156_2_F10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivinghomozygous C57BL/6NTac
Breeding at archiving centreReceived as frozen sperm.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased hematocrit / IMPC
  • decreased mean corpuscular volume / IMPC
  • decreased hemoglobin content / IMPC
  • decreased circulating iron level / IMPC
  • decreased circulating serum albumin level / IMPC
  • thrombocytosis / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • decreased circulating total protein level / IMPC
  • decreased circulating bilirubin level / IMPC
  • increased circulating magnesium level / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating bilirubin level / IMPC
  • decreased hemoglobin content / IMPC
  • decreased circulating iron level / IMPC
  • thrombocytosis / IMPC
  • decreased mean corpuscular volume / IMPC
  • decreased circulating serum albumin level / IMPC
  • decreased circulating total protein level / IMPC
  • increased circulating magnesium level / IMPC
  • decreased hematocrit / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
MGI phenotypes (allele matching)
  • decreased body weight / MGI
  • decreased erythrocyte cell number / MGI
  • decreased circulating iron level / MGI
  • increased circulating magnesium level / MGI
  • decreased hematocrit / MGI
  • anemia / MGI
  • stomach inflammation / MGI
  • decreased mean corpuscular volume / MGI
  • decreased hemoglobin content / MGI
  • abnormal gastric parietal cell morphology / MGI
  • abnormal gastric chief cell morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • hematopoietic system phenotype / MGI
  • thrombocytosis / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • increased stomach pH / MGI
  • hypochromic microcytic anemia / MGI
  • decreased spleen iron level / MGI
  • increased red blood cell distribution width / MGI
  • increased adenoma incidence / MGI
  • increased circulating erythropoietin level / MGI
  • decreased circulating ferritin level / MGI
  • gastric gland hyperplasia / MGI
MGI phenotypes (gene matching)
  • decreased hematocrit / MGI
  • abnormal stomach glandular epithelium morphology / MGI
  • decreased digestive secretion / MGI
  • decreased body weight / MGI
  • anemia / MGI
  • stomach inflammation / MGI
  • decreased mean corpuscular volume / MGI
  • decreased hemoglobin content / MGI
  • decreased erythrocyte cell number / MGI
  • enlarged stomach / MGI
  • abnormal gastric parietal cell morphology / MGI
  • abnormal gastric chief cell morphology / MGI
  • decreased circulating iron level / MGI
  • increased circulating gastrin level / MGI
  • homeostasis/metabolism phenotype / MGI
  • digestive/alimentary phenotype / MGI
  • hematopoietic system phenotype / MGI
  • thrombocytosis / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • hypochlorhydria / MGI
  • achlorhydria / MGI
  • increased stomach pH / MGI
  • hypochromic microcytic anemia / MGI
  • decreased spleen iron level / MGI
  • increased red blood cell distribution width / MGI
  • increased circulating magnesium level / MGI
  • increased adenoma incidence / MGI
  • increased circulating erythropoietin level / MGI
  • decreased circulating ferritin level / MGI
  • gastric gland hyperplasia / MGI
  • abnormal stomach glandular region morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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