C57BL/6NTac-Kcne2^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:05862
Citation information	RRID:IMSR_EM:05862 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Kcne2^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0156_2_F10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Kcne2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Kcne2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0156_2_F10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6NTac males
Breeding at archiving centre	Received as frozen sperm.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Romano-Ward syndrome / Orphanet_101016
Familial atrial fibrillation / Orphanet_334

IMPC phenotypes (allele matching)

decreased hematocrit / IMPC
decreased mean corpuscular volume / IMPC
decreased hemoglobin content / IMPC
decreased circulating iron level / IMPC
decreased circulating serum albumin level / IMPC
thrombocytosis / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased circulating total protein level / IMPC
decreased circulating bilirubin level / IMPC
increased circulating magnesium level / IMPC

IMPC phenotypes (gene matching)

decreased circulating bilirubin level / IMPC
decreased hemoglobin content / IMPC
decreased circulating iron level / IMPC
thrombocytosis / IMPC
decreased mean corpuscular volume / IMPC
decreased circulating serum albumin level / IMPC
decreased circulating total protein level / IMPC
increased circulating magnesium level / IMPC
decreased hematocrit / IMPC
decreased mean corpuscular hemoglobin / IMPC

MGI phenotypes (allele matching)

decreased body weight / MGI
decreased erythrocyte cell number / MGI
decreased circulating iron level / MGI
increased circulating magnesium level / MGI
decreased hematocrit / MGI
anemia / MGI
stomach inflammation / MGI
decreased mean corpuscular volume / MGI
decreased hemoglobin content / MGI
abnormal gastric parietal cell morphology / MGI
abnormal gastric chief cell morphology / MGI
homeostasis/metabolism phenotype / MGI
hematopoietic system phenotype / MGI
thrombocytosis / MGI
decreased mean corpuscular hemoglobin / MGI
increased stomach pH / MGI
hypochromic microcytic anemia / MGI
decreased spleen iron level / MGI
increased red blood cell distribution width / MGI
increased adenoma incidence / MGI
increased circulating erythropoietin level / MGI
decreased circulating ferritin level / MGI
gastric gland hyperplasia / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
abnormal stomach glandular epithelium morphology / MGI
decreased digestive secretion / MGI
decreased body weight / MGI
anemia / MGI
stomach inflammation / MGI
decreased mean corpuscular volume / MGI
decreased hemoglobin content / MGI
decreased erythrocyte cell number / MGI
enlarged stomach / MGI
abnormal gastric parietal cell morphology / MGI
abnormal gastric chief cell morphology / MGI
decreased circulating iron level / MGI
increased circulating gastrin level / MGI
homeostasis/metabolism phenotype / MGI
digestive/alimentary phenotype / MGI
hematopoietic system phenotype / MGI
thrombocytosis / MGI
decreased mean corpuscular hemoglobin / MGI
hypochlorhydria / MGI
achlorhydria / MGI
increased stomach pH / MGI
hypochromic microcytic anemia / MGI
decreased spleen iron level / MGI
increased red blood cell distribution width / MGI
increased circulating magnesium level / MGI
increased adenoma incidence / MGI
increased circulating erythropoietin level / MGI
decreased circulating ferritin level / MGI
gastric gland hyperplasia / MGI
abnormal stomach glandular region morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Kcne2tm1a(EUCOMM)Wtsi/WtsiH