C57BL/6NTac-Wbp2^{tm2a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:05860
International strain name	C57BL/6NTac-Wbp2^{tm2a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0037_2_D06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Wbp2^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Wbp2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0037_2_D06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131 Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing.;Buniello Annalisa, Ingham Neil J, Lewis Morag A, Huma Andreea C, Martinez-Vega Raquel, Varela-Nieto Isabel, Vizcay-Barrena Gema, Fleck Roland A, Houston Oliver, Bardhan Tanaya, Johnson Stuart L, White Jacqueline K, Yuan Huijun, Marcotti Walter, Steel Karen P, ;2016;EMBO molecular medicine;8;191-207; 26881968

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6NTac
Breeding at archiving centre	Received as frozen sperm.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
decreased hematocrit / IMPC
increased leukocyte cell number / IMPC
hyperactivity / IMPC
increased startle reflex / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
abnormal behavior / IMPC
absent pinna reflex / IMPC

IMPC phenotypes (gene matching)

absent pinna reflex / IMPC
decreased erythrocyte cell number / IMPC
increased startle reflex / IMPC
decreased hemoglobin content / IMPC
hyperactivity / IMPC
increased leukocyte cell number / IMPC
decreased bone mineral density / IMPC
decreased hematocrit / IMPC
abnormal behavior / IMPC

MGI phenotypes (allele matching)

abnormal organ of Corti morphology / MGI
abnormal distortion product otoacoustic emission / MGI
sensorineural hearing loss / MGI
abnormal cochlear IHC afferent innervation pattern / MGI
abnormal cochlear OHC afferent innervation pattern / MGI
hearing/vestibular/ear phenotype / MGI
abnormal auditory summating potential / MGI
decreased circulating amylase level / MGI
abnormal auditory brainstem response waveform shape / MGI
increased or absent threshold for auditory brainstem response / MGI
hyperactivity / MGI
abnormal behavior / MGI
decreased circulating serum albumin level / MGI
absent pinna reflex / MGI
decreased circulating fructosamine level / MGI

MGI phenotypes (gene matching)

abnormal organ of Corti morphology / MGI
hyperactivity / MGI
abnormal distortion product otoacoustic emission / MGI
sensorineural hearing loss / MGI
abnormal cochlear IHC afferent innervation pattern / MGI
abnormal cochlear OHC afferent innervation pattern / MGI
abnormal behavior / MGI
hearing/vestibular/ear phenotype / MGI
decreased circulating serum albumin level / MGI
absent pinna reflex / MGI
abnormal auditory summating potential / MGI
decreased circulating amylase level / MGI
decreased circulating fructosamine level / MGI
abnormal auditory brainstem response waveform shape / MGI
increased or absent threshold for auditory brainstem response / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing.;Buniello Annalisa, Ingham Neil J, Lewis Morag A, Huma Andreea C, Martinez-Vega Raquel, Varela-Nieto Isabel, Vizcay-Barrena Gema, Fleck Roland A, Houston Oliver, Bardhan Tanaya, Johnson Stuart L, White Jacqueline K, Yuan Huijun, Marcotti Walter, Steel Karen P, ;2016;EMBO molecular medicine;8;191-207; 26881968

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Wbp2tm2a(EUCOMM)Wtsi/WtsiH