B6Brd;B6N-Tyrc-Brd Pds5btm1a(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:05855
International strain nameB6Brd;B6N-Tyrc-Brd Pds5btm1a(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0028_6_D04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPds5btm1a(EUCOMM)Wtsi
Gene/Transgene symbolPds5b
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0028_6_D04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac
Breeding at archiving centreReceived as frozen materials. Due to the possible presence of C57BL/6J-Tyrc-Brd, may produce albino pups if intercrossed.

Disease and phenotype information

MGI phenotypes (gene matching)
  • delayed bone ossification / MGI
  • short mandible / MGI
  • rib fusion / MGI
  • abnormal heart morphology / MGI
  • decreased cell proliferation / MGI
  • microcephaly / MGI
  • short snout / MGI
  • short limbs / MGI
  • abnormal superior cervical ganglion morphology / MGI
  • abnormal enteric nervous system morphology / MGI
  • cyanosis / MGI
  • decreased brown adipose tissue amount / MGI
  • respiratory distress / MGI
  • abnormal axial skeleton morphology / MGI
  • abnormal craniofacial bone morphology / MGI
  • decreased germ cell number / MGI
  • no phenotypic analysis / MGI
  • abnormal hyoid bone morphology / MGI
  • abnormal cell nucleus morphology / MGI
  • decreased rib number / MGI
  • abnormal chromosome morphology / MGI
  • pallor / MGI
  • abnormal long bone morphology / MGI
  • abnormal facial morphology / MGI
  • aneuploidy / MGI
  • abnormal mitosis / MGI
  • fetal growth retardation / MGI
  • short sternum / MGI
  • short scapula / MGI
  • short humerus / MGI
  • short radius / MGI
  • short ulna / MGI
  • decreased male germ cell number / MGI
  • abnormal vertebrae development / MGI
  • abnormal sternum ossification / MGI
  • abnormal sympathetic postganglionic fiber morphology / MGI
  • decreased birth body size / MGI
  • abnormal palatal shelf fusion at midline / MGI
  • palatal shelves fail to meet at midline / MGI
  • cleft secondary palate / MGI
  • abnormal palate bone morphology / MGI
  • ventricular septal defect / MGI
  • ostium secundum atrial septal defect / MGI
  • complete atrioventricular septal defect / MGI
  • perimembraneous ventricular septal defect / MGI
  • neonatal lethality, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • thin upper lip / MGI
  • abnormal chin morphology / MGI
  • short chin / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

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Legally binding conditions for the transfer

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